Human Osteopontin/OPN ELISA Kit

$350.00$450.00

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TARGETSSPP1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity4.42 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery75% – 126%
Average Recovery0.99
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity4.42 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery75% – 126%
Average Recovery0.99
Intra Precision4.1% – 5.3%
Inter-Precision7.2% – 9.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Osteopontin/OPN monoclonal antibody
Human Osteopontin/OPN freeze-dried standard
Osteopontin/OPN detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human OPN antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the OPN present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of OPN in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

SPP1

SPP1 Target Infomation Overview

  • Target Symbol: SPP1, secreted phosphoprotein 1
  • Gene Groups: Receptor ligands; SIBLING family
  • Alias: BSPI; ETA-1
  • Previous Names: BNSP; OPN
  • Alias Names: early T-lymphocyte activation 1; osteopontin; bone sialoprotein I

SPP1, secreted phosphoprotein 1 Target Infomation by Species

HumanMouseRat

Human SPP1 Target Information

  • Target Symbol: SPP1, secreted phosphoprotein 1
  • Alias:
    • BNSP
    • bone sialoprotein 1
    • bone sialoprotein I
    • BSPI
    • early T-lymphocyte activation 1
    • ETA-1
    • MGC110940
    • nephropontin
    • OPN
    • osteopontin
    • osteopontin-C
    • osteopontin-D
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
    • secreted phosphoprotein 1 variant 6
    • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
    • SPP-1
    • SPP1/CALPHA1 fusion
    • urinary stone protein
    • uropontin
  • NCBI_Gene: 6696
  • UniProtKB: P10451

Human SPP1 Predicted Functions

Enables integrin binding activity. Involved in several processes, including androgen catabolic process; cellular response to testosterone stimulus; and positive regulation of estradiol secretion. Located in Golgi apparatus and extracellular space. Implicated in several diseases, including autoimmune disease (multiple); biliary atresia; coronary artery disease (multiple); disease of cellular proliferation (multiple); and hepatitis. Biomarker of calcinosis; coronary artery disease; kidney failure; progressive osseous heteroplasia; and urinary bladder cancer.

Mouse Spp1 Target Information

Mouse Spp1 Predicted Functions

Enables extracellular matrix binding activity. Acts upstream of or within several processes, including cellular ion homeostasis; cellular response to leukemia inhibitory factor; and neutrophil chemotaxis. Located in apical part of cell and cytoplasm. Is expressed in several structures, including alimentary system; brain; metanephros; reproductive system; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; coronary artery disease (multiple); disease of cellular proliferation (multiple); and hepatitis. Orthologous to human SPP1 (secreted phosphoprotein 1).

Rat Spp1 Target Information

  • Target Symbol: Spp1, secreted phosphoprotein 1
  • Alias:
    • bone sialoprotein 1
    • LOC100359743
    • OSP
    • osteopontin
    • osteopontin-like
    • Sialoprotein (osteopontin)
    • SPP-1
  • NCBI_Gene: 25353
  • UniProtKB: P08721

Rat Spp1 Predicted Functions

Predicted to enable extracellular matrix binding activity and integrin binding activity. Involved in several processes, including negative regulation of collateral sprouting of intact axon in response to injury; osteoblast differentiation; and positive regulation of bone resorption. Located in several cellular components, including extracellular space; perinuclear region of cytoplasm; and vesicle. Used to study several diseases, including encephalomyelitis; myocarditis; radiculopathy; renovascular hypertension; and visual epilepsy. Biomarker of autosomal dominant polycystic kidney disease; calcinosis; and glomerulosclerosis. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; coronary artery disease (multiple); disease of cellular proliferation (multiple); and hepatitis. Orthologous to human SPP1 (secreted phosphoprotein 1).

SPP1 Target News

    5.8
    MIF is a critical regulator of mononuclear phagocytic infiltration in hepatocellular carcinoma
    iScience
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Multiple Sclerosis
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Systemic Lupus Erythematosus
    Tumor Marker

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