Name: Human PDGFRB/CD140b ELISA Kit
SKU: EK1232
Availability: InStock

EK1232

Human PDGFRB/CD140b ELISA Kit

$245.00 – $350.00

Related Target	PDGFRB
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	80 μL 1×Assay Buffer and 20 μL sample
Sensitivity	48.54 pg/mL
Array Range	0.63 ng/mL – 40 ng/mL
Assay Time	3.5 h
Recovery	70% – 117%
Average Recovery	88%

ELISA Kit Detail Information

Related Target	PDGFRB
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	80 μL 1×Assay Buffer and 20 μL sample
Sensitivity	48.54 pg/mL
Array Range	0.63 ng/mL – 40 ng/mL
Assay Time	3.5 h
Recovery	70% – 117%
Average Recovery	88%
Intra Precision	6.1% – 8.7%
Inter Precision	7.0% - 8.2%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PDGFRB/CD140b monoclonal antibody Human PDGFRB/CD140b freeze-dried standard PDGFRB/CD140b detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PDGFRB antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, PDGFRB present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PDGFRB in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

PDGFRB

PDGFRB Target Infomation Overview

Target Symbol: PDGFRB, platelet derived growth factor receptor beta
Gene Groups: Receptor tyrosine kinases; CD molecules; I-set domain containing
Alias: JTK12; CD140b; PDGFR1
Previous Names: PDGFR
Alias Names: platelet-derived growth factor receptor, beta polypeptide

PDGFRB, platelet derived growth factor receptor beta Target Infomation by Species

Human
Mouse
Rat

Human PDGFRB Target Information

Target Symbol: PDGFRB, platelet derived growth factor receptor beta
Alias:
- Activated tyrosine kinase PDGFRB
- beta-type platelet-derived growth factor receptor
- CD140 antigen-like family member B
- CD140B
- IBGC4
- IMF1
- JTK12
- KOGS
- NDEL1-PDGFRB
- PDGF-R-beta
- PDGFR
- PDGFR-1
- PDGFR-beta
- PDGFR1
- PENTT
- platelet-derived growth factor receptor 1
- platelet-derived growth factor receptor beta
- platelet-derived growth factor receptor, beta polypeptide
NCBI_Gene: 5159
UniProtKB: P09619

Human PDGFRB Predicted Functions

Enables several functions, including growth factor binding activity; platelet-derived growth factor beta-receptor activity; and platelet-derived growth factor receptor binding activity. Involved in several processes, including platelet-derived growth factor receptor signaling pathway; positive regulation of phosphate metabolic process; and protein phosphorylation. Located in Golgi apparatus and membrane. Is intrinsic component of plasma membrane. Implicated in basal ganglia calcification; glioblastoma; infantile myofibromatosis; myeloproliferative neoplasm (multiple); and renal cell carcinoma. Biomarker of Kuhnt-Junius degeneration; breast cancer (multiple); renal cell carcinoma; and reproductive organ cancer (multiple).

Mouse Pdgfrb Target Information

Target Symbol: Pdgfrb, platelet derived growth factor receptor, beta polypeptide
Alias:
- AI528809
- CD140b
- expressed sequence AI528809
- Pdgfr
- platelet derived growth factor receptor
NCBI_Gene: 18596

Mouse Pdgfrb Predicted Functions

Enables platelet-derived growth factor-activated receptor activity and signaling receptor binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; protein phosphorylation; and vasculature development. Acts upstream of or within several processes, including animal organ development; response to ceramide; and ruffle assembly. Located in cell surface; cytoplasm; and ruffle. Is expressed in several structures, including alimentary system; extraembryonic component; genitourinary system; nervous system; and sensory organ. Used to study basal ganglia calcification; infantile myofibromatosis; myeloproliferative neoplasm; and severe nonproliferative diabetic retinopathy. Human ortholog(s) of this gene implicated in basal ganglia calcification; glioblastoma; infantile myofibromatosis; myeloproliferative neoplasm (multiple); and renal cell carcinoma. Orthologous to human PDGFRB (platelet derived growth factor receptor beta).

Rat Pdgfrb Target Information

Target Symbol: Pdgfrb, platelet derived growth factor receptor beta
Alias:
- beta platelet-derived growth factor receptor
- beta-type platelet-derived growth factor receptor
- CD140 antigen-like family member B
- PDGF-R-beta
- PDGFR-1
- PDGFR-beta
- platelet derived growth factor receptor, beta polypeptide
- platelet-derived growth factor receptor 1
- Platelet-derived growth factor receptor beta
- platelet-derived growth factor receptor beta variant 1
- Platelet-derived growth factor receptor, beta
NCBI_Gene: 24629
UniProtKB: Q05030

Rat Pdgfrb Predicted Functions

Enables phosphatidylinositol 3-kinase binding activity and platelet-derived growth factor-activated receptor activity. Involved in several processes, including animal organ development; positive regulation of Rho protein signal transduction; and regulation of apoptotic process. Located in several cellular components, including apical plasma membrane; cell surface; and nucleus. Used to study extrahepatic cholestasis; middle cerebral artery infarction; and prostate cancer. Biomarker of hypertension; oligohydramnios; and sciatic neuropathy. Human ortholog(s) of this gene implicated in basal ganglia calcification; glioblastoma; infantile myofibromatosis; myeloproliferative neoplasm (multiple); and renal cell carcinoma. Orthologous to human PDGFRB (platelet derived growth factor receptor beta).

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