Name: Human Pro-Collagen I α1 ELISA Kit
SKU: EK1C01
Availability: InStock

EK1C01

Human Pro-Collagen I α1 ELISA Kit

$245.00 – $350.00

Related Target	col1a1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	0.67 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	72% – 112%
Average Recovery	90%

ELISA Kit Detail Information

Related Target	col1a1
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	0.67 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	72% – 112%
Average Recovery	90%
Intra Precision	2.3% – 3.8%
Inter Precision	1.0% – 1.2%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with mouse anti-human Pro-Collagen Iα capture antibody Human Pro-Collagen Iα1 freeze-dried standard Human Pro-Collagen Iα1 detect Antibody Streptavidin-HRP Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film Standard Diluent
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Pro-COL I A1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the Pro-COL I A1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Pro-COL I A1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

col1a1

No description available.

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