Human Prolactin ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	PRL
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	23.7 pg/mL
Range	0.39 ng/mL – 25 ng/mL
Assay Time	3.5 h
Recovery	85% – 116%
Average Recovery	0.99

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	23.7 pg/mL
Range	0.39 ng/mL – 25 ng/mL
Assay Time	3.5 h
Recovery	85% – 116%
Average Recovery	0.99
Intra Precision	2.5% – 3.5%
Inter-Precision	4.4% – 7.6%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Prolactin monoclonal antibody Human Prolactin freeze-dried standard Prolactin detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Prolactin单克隆 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Prolactin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Prolactin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

PRL

PRL Target Infomation Overview

Target Symbol: PRL, prolactin
Gene Groups: Growth hormone family; Neuropeptides

PRL, prolactin Target Infomation by Species

HumanMouseRat

Human PRL Target Information

Target Symbol: PRL, prolactin
Alias:
- decidual prolactin
- GHA1
- growth hormone A1
NCBI_Gene: 5617
UniProtKB: P01236

Human PRL Predicted Functions

Predicted to enable hormone activity and prolactin receptor binding activity. Involved in several processes, including negative regulation of endothelial cell proliferation; positive regulation of pri-miRNA transcription by RNA polymerase II; and positive regulation of receptor signaling pathway via JAK-STAT. Predicted to be located in secretory granule. Predicted to be active in extracellular space. Implicated in carotid artery disease. Biomarker of hepatocellular carcinoma; hypoglycemia; obesity; and severe acute respiratory syndrome.

Mouse Prl Target Information

Target Symbol: Prl, prolactin
Alias:
- AV290867
- expressed sequence AV290867
- Prl1a1
NCBI_Gene: 19109

Mouse Prl Predicted Functions

Predicted to enable hormone activity and prolactin receptor binding activity. Acts upstream of or within several processes, including mammary gland development; maternal behavior; and positive regulation of receptor signaling pathway via JAK-STAT. Located in secretory granule. Is expressed in hippocampus; pituitary gland; placenta; and trophoblast giant cell. Human ortholog(s) of this gene implicated in carotid artery disease. Orthologous to human PRL (prolactin).

Rat Prl Target Information

Target Symbol: Prl, prolactin
Alias:
- Gha1
- growth hormone a1
- Prl1a1
- PRLB
- PRLSD1
- Prol
- prolactin family 1, subfamily a, member 1
- RATPRLSD1
- RNPROL
NCBI_Gene: 24683

Rat Prl Predicted Functions

Predicted to enable hormone activity and prolactin receptor binding activity. Involved in several processes, including parturition; response to estradiol; and response to lipopolysaccharide. Located in extracellular region and secretory granule. Used to study portal hypertension and prolactinoma. Biomarker of hyperprolactinemia; prolactinoma; and renovascular hypertension. Human ortholog(s) of this gene implicated in carotid artery disease. Orthologous to human PRL (prolactin).