Human sCD14 ELISA Kit

$350.00$450.00

Download:
TARGETSCD14
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity2.58 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery96% – 110%
Average Recovery1.03
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity2.58 pg/mL
Range125 pg/mL – 8000 pg/mL
Assay Time3.5 h
Recovery96% – 110%
Average Recovery1.03
Intra Precision3.2% – 4.8%
Inter-Precision2.7% – 4.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sCD14 monoclonal antibody
Human sCD14 freeze-dried standard
sCD14 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human sCD14 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sCD14 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sCD14 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CD14

CD14 Target Infomation Overview

  • Target Symbol: CD14, CD14 molecule
  • Gene Groups: CD molecules; Scavenger receptors
  • Alias Names: CD14 antigen

CD14, CD14 molecule Target Infomation by Species

HumanMouseRat

Human CD14 Target Information

  • Target Symbol: CD14, CD14 molecule
  • Alias:
    • cd14 antigen
    • monocyte differentiation antigen CD14
    • myeloid cell-specific leucine-rich glycoprotein
  • NCBI_Gene: 929
  • UniProtKB: P08571

Human CD14 Predicted Functions

Enables lipopeptide binding activity; lipopolysaccharide binding activity; and lipoteichoic acid binding activity. Involved in cellular response to bacterial lipopeptide; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interleukin-8 production. Acts upstream of or within cellular response to lipopolysaccharide; cellular response to lipoteichoic acid; and positive regulation of tumor necrosis factor production. Located in Golgi apparatus and membrane raft. Is anchored component of external side of plasma membrane. Part of lipopolysaccharide receptor complex. Implicated in several diseases, including IgA glomerulonephritis; artery disease (multiple); celiac disease; cystic fibrosis; and pulmonary tuberculosis. Biomarker of several diseases, including biliary atresia; cystic fibrosis; lung disease (multiple); perinatal necrotizing enterocolitis; and polycystic kidney disease (multiple).

Mouse Cd14 Target Information

Mouse Cd14 Predicted Functions

Predicted to enable lipopeptide binding activity; lipopolysaccharide binding activity; and lipoteichoic acid binding activity. Involved in several processes, including lipopolysaccharide-mediated signaling pathway; positive regulation of cytokine production; and toll-like receptor 4 signaling pathway. Located in external side of plasma membrane and membrane raft. Is expressed in several structures, including femur; gallbladder; intestine; reproductive system; and telencephalon. Human ortholog(s) of this gene implicated in several diseases, including IgA glomerulonephritis; artery disease (multiple); celiac disease; cystic fibrosis; and perinatal necrotizing enterocolitis. Orthologous to human CD14 (CD14 molecule).

Rat Cd14 Target Information

  • Target Symbol: Cd14, CD14 molecule
  • Alias:
    • CD14 antigen
    • monocyte differentiation antigen CD14
    • myeloid cell-specific leucine-rich glycoprotein
  • NCBI_Gene: 60350
  • UniProtKB: Q63691

Rat Cd14 Predicted Functions

Predicted to enable lipopeptide binding activity; lipopolysaccharide binding activity; and lipoteichoic acid binding activity. Involved in several processes, including cellular response to lipopolysaccharide; response to magnesium ion; and response to tumor necrosis factor. Located in cell surface; extracellular space; and plasma membrane. Used to study perinatal necrotizing enterocolitis. Biomarker of Hirschsprung’s disease; cholestasis; obstructive jaundice; and periodontitis. Human ortholog(s) of this gene implicated in several diseases, including IgA glomerulonephritis; artery disease (multiple); celiac disease; cystic fibrosis; and pulmonary tuberculosis. Orthologous to human CD14 (CD14 molecule).

CD14 Target News

    Sepsis

    Related Products

    more +

    EK1392

    $350.00$450.00

    EK1341

    $350.00$450.00

    EK1C01

    $350.00$450.00

    Same Target