Human sICAM-3/CD50 ELISA Kit

$245.00 – $350.00

Related Target	ICAM3
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	5.09 pg/mL
Array Range	125 pg/mL – 8000 pg/mL
Assay Time	3.5 h
Recovery	77% – 112%
Average Recovery	93%

ELISA Kit Detail Information

Related Target	ICAM3
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	5.09 pg/mL
Array Range	125 pg/mL – 8000 pg/mL
Assay Time	3.5 h
Recovery	77% – 112%
Average Recovery	93%
Intra Precision	2.5% – 3.9%
Inter Precision	1.6% – 6.2%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-17AF monoclonal antibody Human IL-17AF freeze-dried standard IL-17AF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human sICAM-3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, sICAM-3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sICAM-3 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

ICAM3

No description available.

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