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Human Thrombospondin-1/THBS-1 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL |
| Sensitivity | 26.39 pg/mL |
| Range | 0.78 ng/mL – 50 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 85% – 110% |
| Average Recovery | 0.99 |
| Intra Precision | 2.3% – 2.5% |
| Inter-Precision | 3.5% – 5.6% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Thrombospondin-1/THBS-1 monoclonal antibody Human Thrombospondin-1/THBS-1 freeze-dried standard Thrombospondin-1/THBS-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human THBS-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, THBS-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of THBS-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
THBS1
THBS1 Target Infomation Overview
- Target Symbol: THBS1, thrombospondin 1
- Alias: TSP1; THBS; TSP; THBS-1; TSP-1
- Alias Names: thrombospondin-1p180
THBS1, thrombospondin 1 Target Infomation by Species
HumanMouseRat
Human THBS1 Target Information
- Target Symbol: THBS1, thrombospondin 1
- Alias:
- glycoprotein G
- THBS
- THBS-1
- thrombospondin-1
- thrombospondin-1p180
- thrombospondin-p50
- TSP
- TSP-1
- TSP1
- NCBI_Gene: 7057
- UniProtKB: P07996
Human THBS1 Predicted Functions
Enables several functions, including collagen V binding activity; fibrinogen binding activity; and fibroblast growth factor binding activity. Involved in several processes, including regulation of cell migration; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of endothelial cell chemotaxis; negative regulation of endothelial cell proliferation; and negative regulation of nitric oxide mediated signal transduction. Located in several cellular components, including external side of plasma membrane; extracellular space; and platelet alpha granule. Part of fibrinogen complex. Colocalizes with collagen-containing extracellular matrix. Implicated in cholangiocarcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Biomarker of COVID-19; cholangiocarcinoma; obesity; pancreatic cancer (multiple); and urinary bladder cancer.
Mouse Thbs1 Target Information
- Target Symbol: Thbs1, thrombospondin 1
- Alias:
- tbsp1
- Thbs-1
- TSP-1
- TSP1
- NCBI_Gene: 21825
Mouse Thbs1 Predicted Functions
Enables extracellular matrix binding activity. Involved in several processes, including behavioral response to pain; positive regulation of macrophage chemotaxis; and positive regulation of transforming growth factor beta receptor signaling pathway. Acts upstream of or within several processes, including cellular response to nitric oxide; circulatory system development; and monocyte aggregation. Located in extracellular space and sarcoplasmic reticulum. Is expressed in several structures, including alimentary system; brain; genitourinary system; limb; and sensory organ. Used to study Sjogren’s syndrome. Human ortholog(s) of this gene implicated in cholangiocarcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Orthologous to human THBS1 (thrombospondin 1).
Rat Thbs1 Target Information
- Target Symbol: Thbs1, thrombospondin 1
- Alias:
- thrombospondin-1
- TSP-1
- Tsp1
- NCBI_Gene: 445442
Rat Thbs1 Predicted Functions
Enables transforming growth factor beta binding activity. Involved in several processes, including cellular response to tumor necrosis factor; positive regulation of transforming growth factor beta production; and response to testosterone. Located in cytoplasm and extracellular space. Biomarker of diabetic retinopathy; heart disease; myocardial infarction; and prostate cancer. Human ortholog(s) of this gene implicated in cholangiocarcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Orthologous to human THBS1 (thrombospondin 1).
