Human Thrombospondin-1/THBS-1 ELISA Kit

$350.00$450.00

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TARGETSTHBS1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity26.39 pg/mL
Range0.78 ng/mL – 50 ng/mL
Assay Time3.5 h
Recovery85% – 110%
Average Recovery0.99
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity26.39 pg/mL
Range0.78 ng/mL – 50 ng/mL
Assay Time3.5 h
Recovery85% – 110%
Average Recovery0.99
Intra Precision2.3% – 2.5%
Inter-Precision3.5% – 5.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Thrombospondin-1/THBS-1 monoclonal antibody
Human Thrombospondin-1/THBS-1 freeze-dried standard
Thrombospondin-1/THBS-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human THBS-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, THBS-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of THBS-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

THBS1

THBS1 Target Infomation Overview

  • Target Symbol: THBS1, thrombospondin 1
  • Alias: TSP1; THBS; TSP; THBS-1; TSP-1
  • Alias Names: thrombospondin-1p180

THBS1, thrombospondin 1 Target Infomation by Species

HumanMouseRat

Human THBS1 Target Information

  • Target Symbol: THBS1, thrombospondin 1
  • Alias:
    • glycoprotein G
    • THBS
    • THBS-1
    • thrombospondin-1
    • thrombospondin-1p180
    • thrombospondin-p50
    • TSP
    • TSP-1
    • TSP1
  • NCBI_Gene: 7057
  • UniProtKB: P07996

Human THBS1 Predicted Functions

Enables several functions, including collagen V binding activity; fibrinogen binding activity; and fibroblast growth factor binding activity. Involved in several processes, including regulation of cell migration; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of endothelial cell chemotaxis; negative regulation of endothelial cell proliferation; and negative regulation of nitric oxide mediated signal transduction. Located in several cellular components, including external side of plasma membrane; extracellular space; and platelet alpha granule. Part of fibrinogen complex. Colocalizes with collagen-containing extracellular matrix. Implicated in cholangiocarcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Biomarker of COVID-19; cholangiocarcinoma; obesity; pancreatic cancer (multiple); and urinary bladder cancer.

Mouse Thbs1 Target Information

Mouse Thbs1 Predicted Functions

Enables extracellular matrix binding activity. Involved in several processes, including behavioral response to pain; positive regulation of macrophage chemotaxis; and positive regulation of transforming growth factor beta receptor signaling pathway. Acts upstream of or within several processes, including cellular response to nitric oxide; circulatory system development; and monocyte aggregation. Located in extracellular space and sarcoplasmic reticulum. Is expressed in several structures, including alimentary system; brain; genitourinary system; limb; and sensory organ. Used to study Sjogren’s syndrome. Human ortholog(s) of this gene implicated in cholangiocarcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Orthologous to human THBS1 (thrombospondin 1).

Rat Thbs1 Target Information

Rat Thbs1 Predicted Functions

Enables transforming growth factor beta binding activity. Involved in several processes, including cellular response to tumor necrosis factor; positive regulation of transforming growth factor beta production; and response to testosterone. Located in cytoplasm and extracellular space. Biomarker of diabetic retinopathy; heart disease; myocardial infarction; and prostate cancer. Human ortholog(s) of this gene implicated in cholangiocarcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Orthologous to human THBS1 (thrombospondin 1).

THBS1 Target News

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