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Human TIMP-1 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 3.94 pg/mL |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 90% – 105% |
| Average Recovery | 0.94 |
| Intra Precision | 4.9%-5.4% |
| Inter-Precision | 6.4% – 7.3% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TIMP-1A monoclonal antibody Human TIMP-1A freeze-dried standard TIMP-1A detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TIMP-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TIMP-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TIMP-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
TIMP1
TIMP1 Target Infomation Overview
- Target Symbol: TIMP1, TIMP metallopeptidase inhibitor 1
- Gene Groups: Tissue inhibitor of metallopeptidases
- Alias: EPO
- Previous Names: TIMP; CLGI
- Alias Names: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
TIMP1, TIMP metallopeptidase inhibitor 1 Target Infomation by Species
HumanMouseRat
Human TIMP1 Target Information
- Target Symbol: TIMP1, TIMP metallopeptidase inhibitor 1
- Alias:
- CLGI
- collagenase inhibitor
- EPA
- EPO
- erythroid potentiating activity
- erythroid-potentiating activity
- fibroblast collagenase inhibitor
- FLJ90373
- HCI
- metalloproteinase inhibitor 1
- TIMP
- TIMP-1
- tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
- tissue inhibitor of metalloproteinases 1
- NCBI_Gene: 7076
- UniProtKB: P01033
Human TIMP1 Predicted Functions
Enables cytokine activity; metalloendopeptidase inhibitor activity; and zinc ion binding activity. Involved in several processes, including cellular response to UV-A; negative regulation of proteolysis; and negative regulation of trophoblast cell migration. Located in extracellular space. Biomarker of several diseases, including CAKUT2; Down syndrome; artery disease (multiple); carcinoma (multiple); and diabetes mellitus (multiple).
Mouse Timp1 Target Information
- Target Symbol: Timp1, tissue inhibitor of metalloproteinase 1
- Alias:
- Clgi
- MGC:7143
- TIMP-1
- NCBI_Gene: 21857
Mouse Timp1 Predicted Functions
Predicted to enable several functions, including cytokine activity; metalloendopeptidase inhibitor activity; and zinc ion binding activity. Acts upstream of or within connective tissue replacement involved in inflammatory response wound healing. Located in basement membrane. Is expressed in several structures, including early conceptus; hindlimb cartilage condensation; jaw; reproductive system; and skeletal system. Orthologous to human TIMP1 (TIMP metallopeptidase inhibitor 1).
Rat Timp1 Target Information
- Target Symbol: Timp1, TIMP metallopeptidase inhibitor 1
- Alias:
- metalloproteinase inhibitor 1
- Timp
- TIMP-1
- tissue inhibitor of metallopeptidase 1
- tissue inhibitor of metalloproteinase 1
- tissue inhibitor of metalloproteinases 1
- NCBI_Gene: 116510
- UniProtKB: P30120
Rat Timp1 Predicted Functions
Enables protease binding activity. Involved in several processes, including cartilage development; response to peptide hormone; and wound healing. Located in basement membrane. Used to study degenerative disc disease. Biomarker of cholestasis; gastric ulcer; heart disease (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human TIMP1 (TIMP metallopeptidase inhibitor 1).
