Human TIMP-2 ELISA Kit

$350.00$450.00

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TARGETSTIMP2
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.68 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery95% – 119%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.68 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery95% – 119%
Average Recovery1.02
Intra Precision3.8% – 4.1%
Inter-Precision4.2%-4.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TIMP-2 monoclonal antibody
Human TIMP-2 freeze-dried standard
Human TIMP-2 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TIMP-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TIMP-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TIMP-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TIMP2

TIMP2 Target Infomation Overview

  • Target Symbol: TIMP2, TIMP metallopeptidase inhibitor 2
  • Gene Groups: Tissue inhibitor of metallopeptidases
  • Alias: CSC-21K
  • Alias Names: tissue inhibitor of metalloproteinase 2

TIMP2, TIMP metallopeptidase inhibitor 2 Target Infomation by Species

HumanMouseRat

Human TIMP2 Target Information

  • Target Symbol: TIMP2, TIMP metallopeptidase inhibitor 2
  • Alias:
    • CSC-21K
    • DDC8
    • KIAA1731NL
    • metalloproteinase inhibitor 2
    • testicular secretory protein Li 57
    • TIMP-2
    • tissue inhibitor of metalloproteinase 2
    • tissue inhibitor of metalloproteinases 2
  • NCBI_Gene: 7077
  • UniProtKB: P16035

Human TIMP2 Predicted Functions

Enables peptidase inhibitor activity; protease binding activity; and zinc ion binding activity. Involved in negative regulation of metallopeptidase activity. Located in collagen-containing extracellular matrix. Implicated in Moyamoya disease. Biomarker of several diseases, including Duchenne muscular dystrophy; aortic disease (multiple); carcinoma (multiple); neurodegenerative disease (multiple); and preretinal fibrosis.

Mouse Timp2 Target Information

Mouse Timp2 Predicted Functions

Enables enzyme activator activity; integrin binding activity; and metalloendopeptidase inhibitor activity. Acts upstream of or within several processes, including negative regulation of cell population proliferation; regulation of MAPK cascade; and regulation of neuron differentiation. Located in basement membrane and cell surface. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and skeletal system. Human ortholog(s) of this gene implicated in Moyamoya disease. Orthologous to human TIMP2 (TIMP metallopeptidase inhibitor 2).

Rat Timp2 Target Information

  • Target Symbol: Timp2, TIMP metallopeptidase inhibitor 2
  • Alias:
    • metalloproteinase inhibitor 2
    • MGC105282
    • TIMP-2
    • tissue inhibitor of metalloproteinase 2
    • tissue inhibitor of metalloproteinases 2
  • NCBI_Gene: 29543
  • UniProtKB: P30121

Rat Timp2 Predicted Functions

Enables integrin binding activity and metalloendopeptidase inhibitor activity. Involved in several processes, including positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; and regulation of intracellular signal transduction. Located in several cellular components, including cell surface; growth cone; and neuronal cell body. Used to study portal hypertension. Biomarker of cardiomyopathy; congestive heart failure; and intracranial aneurysm. Human ortholog(s) of this gene implicated in Moyamoya disease. Orthologous to human TIMP2 (TIMP metallopeptidase inhibitor 2).

TIMP2 Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Osteoarthrosis & Rheumatoid Arthritis
    Tumor Marker

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