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Human TIMP-2 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 0.68 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 95% – 119% |
| Average Recovery | 1.02 |
| Intra Precision | 3.8% – 4.1% |
| Inter-Precision | 4.2%-4.6% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TIMP-2 monoclonal antibody Human TIMP-2 freeze-dried standard Human TIMP-2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TIMP-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TIMP-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TIMP-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
TIMP2
TIMP2 Target Infomation Overview
- Target Symbol: TIMP2, TIMP metallopeptidase inhibitor 2
- Gene Groups: Tissue inhibitor of metallopeptidases
- Alias: CSC-21K
- Alias Names: tissue inhibitor of metalloproteinase 2
TIMP2, TIMP metallopeptidase inhibitor 2 Target Infomation by Species
HumanMouseRat
Human TIMP2 Target Information
- Target Symbol: TIMP2, TIMP metallopeptidase inhibitor 2
- Alias:
- CSC-21K
- DDC8
- KIAA1731NL
- metalloproteinase inhibitor 2
- testicular secretory protein Li 57
- TIMP-2
- tissue inhibitor of metalloproteinase 2
- tissue inhibitor of metalloproteinases 2
- NCBI_Gene: 7077
- UniProtKB: P16035
Human TIMP2 Predicted Functions
Enables peptidase inhibitor activity; protease binding activity; and zinc ion binding activity. Involved in negative regulation of metallopeptidase activity. Located in collagen-containing extracellular matrix. Implicated in Moyamoya disease. Biomarker of several diseases, including Duchenne muscular dystrophy; aortic disease (multiple); carcinoma (multiple); neurodegenerative disease (multiple); and preretinal fibrosis.
Mouse Timp2 Target Information
- Target Symbol: Timp2, tissue inhibitor of metalloproteinase 2
- Alias:
- D11Bwg1104e
- DNA segment, Chr 11, Brigham & Women’s Genetics 1104 expressed
- Timp-2
- TIMP-2
- NCBI_Gene: 21858
Mouse Timp2 Predicted Functions
Enables enzyme activator activity; integrin binding activity; and metalloendopeptidase inhibitor activity. Acts upstream of or within several processes, including negative regulation of cell population proliferation; regulation of MAPK cascade; and regulation of neuron differentiation. Located in basement membrane and cell surface. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and skeletal system. Human ortholog(s) of this gene implicated in Moyamoya disease. Orthologous to human TIMP2 (TIMP metallopeptidase inhibitor 2).
Rat Timp2 Target Information
- Target Symbol: Timp2, TIMP metallopeptidase inhibitor 2
- Alias:
- metalloproteinase inhibitor 2
- MGC105282
- TIMP-2
- tissue inhibitor of metalloproteinase 2
- tissue inhibitor of metalloproteinases 2
- NCBI_Gene: 29543
- UniProtKB: P30121
Rat Timp2 Predicted Functions
Enables integrin binding activity and metalloendopeptidase inhibitor activity. Involved in several processes, including positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; and regulation of intracellular signal transduction. Located in several cellular components, including cell surface; growth cone; and neuronal cell body. Used to study portal hypertension. Biomarker of cardiomyopathy; congestive heart failure; and intracranial aneurysm. Human ortholog(s) of this gene implicated in Moyamoya disease. Orthologous to human TIMP2 (TIMP metallopeptidase inhibitor 2).
