Human TLR2 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	TLR2
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	3.07 pg/mL
Range	109.38 pg/mL – 7000 pg/mL
Assay Time	3.5 h
Recovery	86% – 116%
Average Recovery	0.94

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	3.07 pg/mL
Range	109.38 pg/mL – 7000 pg/mL
Assay Time	3.5 h
Recovery	86% – 116%
Average Recovery	0.94
Intra Precision	1.1% – 1.4%
Inter-Precision	1.2%-1.9%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TLR2 monoclonal antibody Human TLR2 freeze-dried standard TLR2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TLR2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TLR2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TLR2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

TLR2

TLR2 Target Infomation Overview

Target Symbol: TLR2, toll like receptor 2
Gene Groups: CD molecules; Toll like receptors
Alias: TIL4; CD282
Alias Names: toll-like receptor 2

TLR2, toll like receptor 2 Target Infomation by Species

HumanMouseRat

Human TLR2 Target Information

Target Symbol: TLR2, toll like receptor 2
Alias:
- CD282
- TIL4
- toll-like receptor 2
- toll/interleukin 1 receptor-like 4
- toll/interleukin-1 receptor-like protein 4
NCBI_Gene: 7097
UniProtKB: O60603

Human TLR2 Predicted Functions

Enables several functions, including lipopolysaccharide binding activity; peptide binding activity; and peptidoglycan binding activity. Involved in several processes, including defense response to other organism; positive regulation of cytokine production; and positive regulation of signal transduction. Acts upstream of or within positive regulation of NF-kappaB transcription factor activity and response to molecule of bacterial origin. Located in several cellular components, including Golgi apparatus; cell surface; and membrane raft. Is intrinsic component of plasma membrane. Part of Toll-like receptor 1-Toll-like receptor 2 protein complex. Is integral component of plasma membrane. Implicated in several diseases, including autoimmune disease (multiple); dermatitis (multiple); gastrointestinal system cancer (multiple); lung disease (multiple); and rhinitis (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); middle ear disease (multiple); and nose disease (multiple).

Mouse Tlr2 Target Information

Target Symbol: Tlr2, toll-like receptor 2
Alias:
- Ly105
NCBI_Gene: 24088

Mouse Tlr2 Predicted Functions

Enables lipoteichoic acid binding activity and peptide binding activity. Involved in several processes, including negative regulation of cellular component organization; positive regulation of cytokine production; and positive regulation of macromolecule biosynthetic process. Acts upstream of or within several processes, including pattern recognition receptor signaling pathway; regulation of cytokine production; and response to bacterium. Located in external side of plasma membrane. Part of Toll-like receptor 2-Toll-like receptor 6 protein complex. Is expressed in several structures, including brain; early embryo; genitourinary system; gut; and immune system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); dermatitis (multiple); gastrointestinal system cancer (multiple); lung disease (multiple); and rhinitis (multiple). Orthologous to human TLR2 (toll like receptor 2).

Rat Tlr2 Target Information

Target Symbol: Tlr2, toll-like receptor 2
Alias:
- toll-like receptor 2 variant 1
- toll-like receptor 2 variant 2
NCBI_Gene: 310553
- UniprotKB: E9PTD9
- UniprotKB: Q6YGU2

Rat Tlr2 Predicted Functions

Enables lipopeptide binding activity. Involved in several processes, including neurogenesis; positive regulation of interleukin-10 production; and response to molecule of bacterial origin. Located in cell body; cell projection; and cell surface. Used to study carotid stenosis; ileitis; keratitis; lung disease (multiple); and renal fibrosis. Biomarker of acute necrotizing pancreatitis; kidney failure; lung disease; pancreatitis; and perinatal necrotizing enterocolitis. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); dermatitis (multiple); gastrointestinal system cancer (multiple); lung disease (multiple); and rhinitis (multiple). Orthologous to human TLR2 (toll like receptor 2).