EK1201

Human Transferrin/TF ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity2.82 pg/mL
Array Range0.94 ng/mL – 60 ng/mL
Assay Time3.5 h
Recovery90% – 118%
Average Recovery109%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity2.82 pg/mL
Array Range0.94 ng/mL – 60 ng/mL
Assay Time3.5 h
Recovery90% – 118%
Average Recovery109%
Intra Precision1.8% – 2.6%
Inter Precision1.9% – 3.6%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Transferrin/TF monoclonal antibody
Human Transferrin/TF freeze-dried standard
Transferrin/TF detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TF antibodies are precoated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate for incubation. After washing to remove unbound substances, the detection antibody labeled with horseradish peroxidase is added for further incubation. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

TF

TF Target Infomation Overview

  • Target Symbol: TF, transferrin
  • Gene Groups: Transferrins
  • Alias: PRO1557; PRO2086
  • Alias Names: serotransferrin

TF, transferrin Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human TF Target Information

  • Target Symbol: TF, transferrin
  • Alias:
    • beta-1 metal-binding globulin
    • DKFZp781D0156
    • epididymis secretory sperm binding protein Li 71p
    • HEL-S-71p
    • PRO1557
    • PRO2086
    • serotransferrin
    • siderophilin
    • TFQTL1
  • NCBI_Gene: 7018
  • UniProtKB: P02787

Human TF Predicted Functions

Enables ferrous iron binding activity; iron chaperone activity; and transferrin receptor binding activity. Involved in cellular response to iron ion; positive regulation of receptor-mediated endocytosis; and regulation of iron ion transport. Located in several cellular components, including basal plasma membrane; clathrin-coated pit; and cytoplasmic vesicle. Is extrinsic component of external side of plasma membrane. Part of HFE-transferrin receptor complex. Implicated in anemia; atransferrinemia; glucose metabolism disease (multiple); iron deficiency anemia; and restless legs syndrome. Biomarker of glomerulonephritis; hypertension; and membranous glomerulonephritis.

Mouse Trf Target Information

Mouse Trf Predicted Functions

Predicted to enable iron chaperone activity; iron ion binding activity; and transferrin receptor binding activity. Involved in several processes, including ERK1 and ERK2 cascade; osteoclast differentiation; and positive regulation of bone resorption. Acts upstream of or within SMAD protein signal transduction; iron ion transport; and response to bacterium. Located in clathrin-coated pit and cytoplasmic vesicle. Is expressed in several structures, including brain; liver; placenta; white fat; and yolk sac. Used to study atransferrinemia. Human ortholog(s) of this gene implicated in anemia; atransferrinemia; glucose metabolism disease (multiple); iron deficiency anemia; and restless legs syndrome. Orthologous to human TF (transferrin).

Rat Tf Target Information

Rat Tf Predicted Functions

Enables ferric iron binding activity. Involved in several processes, including cellular response to cAMP; cellular response to follicle-stimulating hormone stimulus; and regulation of nervous system development. Located in basement membrane; cell tip; and extracellular space. Used to study hepatitis. Biomarker of anemia and obesity. Human ortholog(s) of this gene implicated in anemia; atransferrinemia; glucose metabolism disease (multiple); iron deficiency anemia; and restless legs syndrome. Orthologous to human TF (transferrin).

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