Human uPAR ELISA Kit

$245.00 – $350.00

Related Target	PLAUR
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.87 pg/mL
Array Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	87% – 114%
Average Recovery	101%

ELISA Kit Detail Information

Related Target	PLAUR
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	0.87 pg/mL
Array Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	87% – 114%
Average Recovery	101%
Intra Precision	3.0% – 4.7%
Inter Precision	2.1% – 5.0%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-uPAR monoclonal antibody Human uPAR freeze-dried standard uPAR detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human uPAR antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, uPAR present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of uPAR in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

PLAUR

PLAUR Target Infomation Overview

Target Symbol: PLAUR, plasminogen activator, urokinase receptor
Gene Groups: CD molecules; LY6/PLAUR domain containing
Alias: URKR; UPAR; CD87
Alias Names: urokinase-type plasminogen activator (uPA) receptor; urokinase plasminogen activator surface receptor

PLAUR, plasminogen activator, urokinase receptor Target Infomation by Species

Human
Mouse
Rat

Human PLAUR Target Information

Target Symbol: PLAUR, plasminogen activator, urokinase receptor
Alias:
- CD87
- monocyte activation antigen Mo3
- U-PAR
- u-plasminogen activator receptor form 2
- UPAR
- URKR
- urokinase plasminogen activator surface receptor
- urokinase-type plasminogen activator (uPA) receptor
NCBI_Gene: 5329
UniProtKB: Q03405

Human PLAUR Predicted Functions

Enables enzyme binding activity; protein domain specific binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of apoptotic signaling pathway; positive regulation of epidermal growth factor receptor signaling pathway; and positive regulation of release of cytochrome c from mitochondria. Located in cell surface and plasma membrane. Implicated in rheumatoid arthritis. Biomarker of several diseases, including Lyme disease; cardiovascular system disease (multiple); end stage renal disease; hepatitis B; and melioidosis.

Mouse Plaur Target Information

Target Symbol: Plaur, plasminogen activator, urokinase receptor
Alias:
- Cd87
- u-PAR
- uPAR
- urokinase-type plasminogen activator receptor
NCBI_Gene: 18793

Mouse Plaur Predicted Functions

Predicted to enable enzyme binding activity; protein domain specific binding activity; and signaling receptor binding activity. Predicted to be involved in several processes, including negative regulation of apoptotic signaling pathway; positive regulation of epidermal growth factor receptor signaling pathway; and positive regulation of release of cytochrome c from mitochondria. Is integral component of plasma membrane. Is expressed in several structures, including decidua; early conceptus; and spleen. Human ortholog(s) of this gene implicated in rheumatoid arthritis. Orthologous to human PLAUR (plasminogen activator, urokinase receptor).

Rat Plaur Target Information

Target Symbol: Plaur, plasminogen activator, urokinase receptor
Alias:
- Par
- plasminogen activator urokinase receptor 3
- plasminogen activator, urokinase receptor 3
- Plaur3
- U-PAR
- uPAR
- uPAR-2
- uPAR-3
- urinary plasminogen activator receptor 2
- urinary plasminogen activator receptor 3
- urokinase plasminogen activator receptor
- urokinase plasminogen activator surface receptor
NCBI_Gene: 50692

Rat Plaur Predicted Functions

Predicted to enable enzyme binding activity; protein domain specific binding activity; and signaling receptor binding activity. Involved in several processes, including epithelial cell differentiation involved in prostate gland development; skeletal muscle tissue regeneration; and spermatogenesis. Predicted to be located in cell surface. Predicted to be integral component of plasma membrane. Predicted to be active in plasma membrane. Biomarker of chronic obstructive pulmonary disease; myocardial infarction; and status epilepticus. Human ortholog(s) of this gene implicated in rheumatoid arthritis. Orthologous to human PLAUR (plasminogen activator, urokinase receptor).

Citations for Human uPAR ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.