Human VEGF-D ELISA Kit

$350.00$450.00

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TARGETSVEGFD
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity2.43 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery80% – 112%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity2.43 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery80% – 112%
Average Recovery0.97
Intra Precision1.6% – 2.5%
Inter-Precision2.8% – 3.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-VEGF-D monoclonal antibody
Human VEGF-D freeze-dried standard
VEGF-D detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human VEGF-D antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, VEGF-D present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of VEGF-D in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

VEGFD

VEGFD Target Infomation Overview

  • Target Symbol: VEGFD, vascular endothelial growth factor D
  • Gene Groups: VEGF family
  • Alias: VEGF-D
  • Previous Names: FIGF
  • Alias Names: c-fos induced growth factor (vascular endothelial growth factor D)

VEGFD, vascular endothelial growth factor D Target Infomation by Species

HumanMouseRat

Human VEGFD Target Information

  • Target Symbol: VEGFD, vascular endothelial growth factor D
  • Alias:
    • c-fos induced growth factor
    • c-fos induced growth factor (vascular endothelial growth factor D)
    • c-fos-induced growth factor
    • FIGF
    • VEGF-D
  • NCBI_Gene: 2277
  • UniProtKB: O43915

Human VEGFD Predicted Functions

Enables chemoattractant activity and vascular endothelial growth factor receptor binding activity. Involved in induction of positive chemotaxis and positive regulation of mast cell chemotaxis. Predicted to be located in extracellular region and platelet alpha granule lumen. Predicted to be active in extracellular space. Biomarker of ovarian carcinoma and urinary bladder cancer.

Mouse Vegfd Target Information

Mouse Vegfd Predicted Functions

Predicted to enable fibroblast growth factor receptor binding activity and growth factor activity. Involved in positive regulation of cold-induced thermogenesis. Acts upstream of or within positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway and positive regulation of cell population proliferation. Located in extracellular space. Is expressed in several structures, including alimentary system; cerebellum; integumental system; limb; and skeleton. Orthologous to human FGF21 (fibroblast growth factor 21).

Rat Vegfd Target Information

Rat Vegfd Predicted Functions

Enables vascular endothelial growth factor receptor binding activity. Involved in several processes, including positive regulation of interleukin-6 production; regulation of vascular endothelial growth factor receptor signaling pathway; and response to interleukin-1. Predicted to be located in extracellular space. Orthologous to human VEGFD (vascular endothelial growth factor D).

VEGFD Target News

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