Human Vitamin D BP ELISA Kit

$350.00$450.00

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TARGETSGC
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity40.15 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery95% – 114%
Average Recovery1.05
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity40.15 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery95% – 114%
Average Recovery1.05
Intra Precision4.4% – 5.0%
Inter-Precision4.0% – 7.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Vitamin D BP monoclonal antibody
Human Vitamin D BP freeze-dried standard
Vitamin D BP detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Vitamin D BP antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Vitamin D BP present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Vitamin D BP in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

GC

GC Target Infomation Overview

  • Target Symbol: GC, GC vitamin D binding protein
  • Alias: DBP; VDBP; hDBP
  • Alias Names: group-specific component (vitamin D binding protein); GC, vitamin D binding protein

GC, GC vitamin D binding protein Target Infomation by Species

HumanMouseRat

Human GC Target Information

  • Target Symbol: GC, GC vitamin D binding protein
  • Alias:
    • DBP
    • DBP-maf
    • DBP/GC
    • epididymis secretory protein Li 51
    • gc protein-derived macrophage activating factor
    • gc-globulin
    • Gc-MAF
    • GcMAF
    • GRD3
    • group-specific component (vitamin D binding protein)
    • HEL-S-51
    • VDB
    • VDBG
    • VDBP
    • vitamin D-binding alpha-globulin
    • vitamin D-binding protein
    • vitamin D-binding protein-macrophage activating factor
  • NCBI_Gene: 2638
  • UniProtKB: P02774

Human GC Predicted Functions

Enables actin binding activity and calcidiol binding activity. Predicted to be involved in vitamin transport. Predicted to act upstream of or within vitamin D metabolic process. Located in blood microparticle and extracellular exosome. Implicated in several diseases, including autoimmune disease (multiple); chronic obstructive pulmonary disease; hepatocellular carcinoma; human immunodeficiency virus infectious disease; and rheumatic fever. Biomarker of several diseases, including autoimmune disease (multiple); dengue disease; diabetic retinopathy; hepatic encephalopathy; and sciatic neuropathy.

Mouse Gc Target Information

Mouse Gc Predicted Functions

Predicted to enable actin binding activity and calcidiol binding activity. Acts upstream of or within vitamin D metabolic process. Predicted to be located in several cellular components, including axon; extracellular space; and perinuclear region of cytoplasm. Predicted to be active in cytoplasm. Is expressed in several structures, including gut; hemolymphoid system gland; liver; male reproductive gland or organ; and trigeminal nerve. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); chronic obstructive pulmonary disease; hepatocellular carcinoma; human immunodeficiency virus infectious disease; and rheumatic fever. Orthologous to human GC (GC vitamin D binding protein).

Rat Gc Target Information

  • Target Symbol: Gc, GC, vitamin D binding protein
  • Alias:
    • DBP
    • DBP02
    • gc-globulin
    • group specific component
    • group-specific component
    • Group-specific component (vitamin D-binding protein)
    • RATDBP02
    • VDB
    • Vdbp
    • vitamin D-binding protein
  • NCBI_Gene: 24384

Rat Gc Predicted Functions

Enables vitamin D binding activity. Involved in several processes, including female pregnancy; lactation; and response to estradiol. Located in several cellular components, including axon; extracellular space; and perinuclear region of cytoplasm. Biomarker of brain ischemia and hepatocellular carcinoma. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); chronic obstructive pulmonary disease; hepatocellular carcinoma; human immunodeficiency virus infectious disease; and rheumatic fever. Orthologous to human GC (GC vitamin D binding protein).

GC Target News

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