Human Vitamin D BP ELISA Kit

$245.00 – $350.00

Related Target	GC
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	40.15 pg/mL
Array Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	95% – 114%
Average Recovery	105%

ELISA Kit Detail Information

Related Target	GC
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	40.15 pg/mL
Array Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	95% – 114%
Average Recovery	105%
Intra Precision	4.4% – 5.0%
Inter Precision	4.0% – 7.5%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Vitamin D BP monoclonal antibody Human Vitamin D BP freeze-dried standard Vitamin D BP detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Vitamin D BP antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Vitamin D BP present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Vitamin D BP in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

GC

GC Target Infomation Overview

Target Symbol: GC, GC vitamin D binding protein
Alias: DBP; VDBP; hDBP
Alias Names: group-specific component (vitamin D binding protein); GC, vitamin D binding protein

GC, GC vitamin D binding protein Target Infomation by Species

Human
Mouse
Rat

Human GC Target Information

Target Symbol: GC, GC vitamin D binding protein
Alias:
- DBP
- DBP-maf
- DBP/GC
- epididymis secretory protein Li 51
- gc protein-derived macrophage activating factor
- gc-globulin
- Gc-MAF
- GcMAF
- GRD3
- group-specific component (vitamin D binding protein)
- HEL-S-51
- VDB
- VDBG
- VDBP
- vitamin D-binding alpha-globulin
- vitamin D-binding protein
- vitamin D-binding protein-macrophage activating factor
NCBI_Gene: 2638
UniProtKB: P02774

Human GC Predicted Functions

Enables actin binding activity and calcidiol binding activity. Predicted to be involved in vitamin transport. Predicted to act upstream of or within vitamin D metabolic process. Located in blood microparticle and extracellular exosome. Implicated in several diseases, including autoimmune disease (multiple); chronic obstructive pulmonary disease; hepatocellular carcinoma; human immunodeficiency virus infectious disease; and rheumatic fever. Biomarker of several diseases, including autoimmune disease (multiple); dengue disease; diabetic retinopathy; hepatic encephalopathy; and sciatic neuropathy.

Mouse Gc Target Information

Target Symbol: Gc, vitamin D binding protein
Alias:
- DBP
NCBI_Gene: 14473
- UniprotKB: P21614
- UniprotKB: A0A0G2JGM6

Mouse Gc Predicted Functions

Predicted to enable actin binding activity and calcidiol binding activity. Acts upstream of or within vitamin D metabolic process. Predicted to be located in several cellular components, including axon; extracellular space; and perinuclear region of cytoplasm. Predicted to be active in cytoplasm. Is expressed in several structures, including gut; hemolymphoid system gland; liver; male reproductive gland or organ; and trigeminal nerve. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); chronic obstructive pulmonary disease; hepatocellular carcinoma; human immunodeficiency virus infectious disease; and rheumatic fever. Orthologous to human GC (GC vitamin D binding protein).

Rat Gc Target Information

Target Symbol: Gc, GC, vitamin D binding protein
Alias:
- DBP
- DBP02
- gc-globulin
- group specific component
- group-specific component
- Group-specific component (vitamin D-binding protein)
- RATDBP02
- VDB
- Vdbp
- vitamin D-binding protein
NCBI_Gene: 24384
- UniprotKB: P04276
- UniprotKB: Q68FY4

Rat Gc Predicted Functions

Enables vitamin D binding activity. Involved in several processes, including female pregnancy; lactation; and response to estradiol. Located in several cellular components, including axon; extracellular space; and perinuclear region of cytoplasm. Biomarker of brain ischemia and hepatocellular carcinoma. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); chronic obstructive pulmonary disease; hepatocellular carcinoma; human immunodeficiency virus infectious disease; and rheumatic fever. Orthologous to human GC (GC vitamin D binding protein).

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