Mouse CCL22/MDC ELISA Kit

$350.00$450.00

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TARGETSCCL22
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity4.07 pg/mL
Range31.25 pg/mL – 2,000 pg/mL
Assay Time3.5 h
Recovery76% – 92%
Average Recovery0.84
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity4.07 pg/mL
Range31.25 pg/mL – 2,000 pg/mL
Assay Time3.5 h
Recovery76% – 92%
Average Recovery0.84
Intra Precision3.4% – 5.1%
Inter-Precision3.6% – 5.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Mouse CCL22/MDC monoclonal antibody
Mouse CCL22/MDC freeze-dried standard
Mouse CCL22/MDC detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse CCL22 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CCL22 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL22 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CCL22

CCL22 Target Infomation Overview

  • Target Symbol: CCL22, C-C motif chemokine ligand 22
  • Gene Groups: Chemokine ligands
  • Alias: MDC; STCP-1; ABCD-1; DC/B-CK; A-152E5.1; MGC34554
  • Previous Names: SCYA22
  • Alias Names: small inducible cytokine subfamily A (Cys-Cys), member 22; chemokine (C-C motif) ligand 22

CCL22, C-C motif chemokine ligand 22 Target Infomation by Species

HumanMouseRat

Human CCL22 Target Information

  • Target Symbol: CCL22, C-C motif chemokine ligand 22
  • Alias:
    • A-152E5.1
    • ABCD-1
    • C-C motif chemokine 22
    • CC chemokine STCP-1
    • chemokine (C-C motif) ligand 22
    • DC/B-CK
    • macrophage-derived chemokine
    • MDC
    • MDC(1-69)
    • MGC34554
    • SCYA22
    • small inducible cytokine A22
    • small inducible cytokine subfamily A (Cys-Cys), member 22
    • small-inducible cytokine A22
    • STCP-1
    • stimulated T cell chemotactic protein 1
    • stimulated T-cell chemotactic protein 1
  • NCBI_Gene: 6367
  • UniProtKB: O00626

Human CCL22 Predicted Functions

Predicted to enable CCR chemokine receptor binding activity and chemokine activity. Involved in antimicrobial humoral immune response mediated by antimicrobial peptide and killing of cells of other organism. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Biomarker of several diseases, including arthritis (multiple); cervical squamous cell carcinoma; lung disease (multiple); pleural tuberculosis; and rhinitis.

Mouse Ccl22 Target Information

Mouse Ccl22 Predicted Functions

Predicted to enable CCR chemokine receptor binding activity and chemokine activity. Predicted to be involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and positive regulation of ERK1 and ERK2 cascade. Predicted to act upstream of or within chemotaxis. Located in extracellular space. Is expressed in inguinal lymph node; thymus; and thymus primordium. Orthologous to human CCL22 (C-C motif chemokine ligand 22).

Rat Ccl22 Target Information

  • Target Symbol: Ccl22, C-C motif chemokine ligand 22
  • Alias:
    • C-C motif chemokine 22
    • chemokine (C-C motif) ligand 22
    • Mdc
    • MGC108943
    • Scya22
    • small inducible cytokine A22
    • small inducible cytokine subfamily A (Cys-Cys) member 22
    • small inducible cytokine subfamily A (Cys-Cys), member 22
  • NCBI_Gene: 117551
  • UniProtKB: Q5I0L5

Rat Ccl22 Predicted Functions

Predicted to enable CCR chemokine receptor binding activity and chemokine activity. Predicted to be involved in several processes, including cellular response to cytokine stimulus; leukocyte chemotaxis; and positive regulation of ERK1 and ERK2 cascade. Predicted to be active in extracellular space. Used to study anti-basement membrane glomerulonephritis. Biomarker of asthma; pneumonia; and pulmonary fibrosis. Orthologous to human CCL22 (C-C motif chemokine ligand 22).

CCL22 Target News

    Allergic Reaction
    Lung Disease (Asthma & COPD)

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