Mouse CCL5/RANTES ELISA Kit

$350.00$450.00

TARGETS CCL5
SPECIES Mouse
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 0.12 pg/mL
Range 15.63 pg/mL – 1000 pg/mL
Assay Time 3.5 h
Recovery 80% – 113%
Average Recovery 0.96
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 20 μL
Sensitivity 0.12 pg/mL
Range 15.63 pg/mL – 1000 pg/mL
Assay Time 3.5 h
Recovery 80% – 113%
Average Recovery 0.96
Intra Precision 2.6% – 3.9%
Inter-Precision 3.0% – 5.8%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL5/RANTES monoclonal antibody
Mouse CCL5/RANTES freeze-dried standard
CCL5/RANTES detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse CCL5 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CCL5 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL5 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CCL5

CCL5 Target Infomation Overview

  • Target Symbol: CCL5, C-C motif chemokine ligand 5
  • Gene Groups: Chemokine ligands
  • Alias: RANTES; SISd; TCP228; MGC17164
  • Previous Names: D17S136E; SCYA5
  • Alias Names: T-cell specific protein p288; T-cell specific RANTES protein; SIS-delta; regulated upon activation, normally T-expressed, and presumably secreted; beta-chemokine RANTES; small inducible cytokine subfamily A (Cys-Cys), member 5; small inducible cytokine A5 (RANTES); chemokine (C-C motif) ligand 5

CCL5, C-C motif chemokine ligand 5 Target Infomation by Species

[su_tabs][su_tab title=”Human” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-human”]

Human CCL5 Target Information

  • Target Symbol: CCL5, C-C motif chemokine ligand 5
  • Alias:
    • beta-chemokine RANTES
    • C-C motif chemokine 5
    • chemokine (C-C motif) ligand 5
    • D17S136E
    • eoCP
    • eosinophil chemotactic cytokine
    • MGC17164
    • RANTES
    • regulated upon activation, normally T-expressed, and presumably secreted
    • SCYA5
    • SIS-delta
    • SISd
    • small inducible cytokine A5 (RANTES)
    • small inducible cytokine subfamily A (Cys-Cys), member 5
    • small-inducible cytokine A5
    • t cell-specific protein P228
    • T-cell specific protein p288
    • T-cell-specific protein RANTES
    • TCP228
  • NCBI_Gene: 6352
  • UniProtKB: P13501

Human CCL5 Predicted Functions

Enables several functions, including enzyme activator activity; phosphatidylinositol phospholipase C activity; and signaling receptor binding activity. Involved in several processes, including cellular response to cytokine stimulus; positive regulation of cell migration; and positive regulation of cell-cell adhesion. Acts upstream of or within positive regulation of T cell migration. Predicted to be located in cytoplasm. Predicted to be active in extracellular space. Implicated in several diseases, including hepatitis B; hepatitis C; liver disease (multiple); pulmonary tuberculosis; and severe acute respiratory syndrome. Biomarker of several diseases, including fatty liver disease (multiple); glucose metabolism disease (multiple); hypertension (multiple); liver cirrhosis (multiple); and lung disease (multiple).

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[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Ccl5 Target Information

Mouse Ccl5 Predicted Functions

Enables CCR1 chemokine receptor binding activity. Involved in negative regulation of macrophage apoptotic process and positive regulation of monocyte chemotaxis. Acts upstream of or within inflammatory response; positive regulation of epithelial cell proliferation; and response to tumor necrosis factor. Located in cytoplasm and extracellular space. Is expressed in several structures, including central nervous system; gonad; hemolymphoid system; late tubule; and retina. Human ortholog(s) of this gene implicated in several diseases, including hepatitis B; hepatitis C; liver disease (multiple); pulmonary tuberculosis; and severe acute respiratory syndrome. Orthologous to human CCL5 (C-C motif chemokine ligand 5).

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[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Ccl5 Target Information

  • Target Symbol: Ccl5, C-C motif chemokine ligand 5
  • Alias:
    • C-C motif chemokine 5
    • chemokine (C-C motif) ligand 5
    • RANTES
    • regulated upon activation normal T-cell expressed and secreted
    • Scya5
    • SIS-delta
    • small inducible cytokine A5
    • small inducible cytokine A5 (RANTES)
    • small-inducible cytokine A5
    • T-cell-specific protein RANTES
  • NCBI_Gene: 81780

Rat Ccl5 Predicted Functions

Enables chemokine activity and heparin binding activity. Involved in several processes, including cellular response to organonitrogen compound; lymphocyte migration; and positive regulation of leukocyte chemotaxis. Located in extracellular space. Used to study Chagas disease; bronchiolitis obliterans; kidney disease; non-alcoholic fatty liver disease; and orchitis. Biomarker of several diseases, including alcoholic hepatitis; artery disease (multiple); cardiac arrest; lung disease (multiple); and uveitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including hepatitis B; hepatitis C; liver disease (multiple); pulmonary tuberculosis; and severe acute respiratory syndrome. Orthologous to human CCL5 (C-C motif chemokine ligand 5).

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CCL5 Target News

[catlist tags=”ccl5″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

5.2
Comprehensive immune landscape of lung-resident memory CD8+ T cells after influenza infection and reinfection in a mouse model
Frontiers in Microbiology
14.978
Hypoxia-Inducible Factor 2α Attenuates Renal Ischemia-Reperfusion Injury by Suppressing CD36-Mediated Lipid Accumulation in Dendritic Cells in a Mouse Model
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
3.6
IGF2BP3 Enhances the Growth of Hepatocellular Carcinoma Tumors by Regulating the Properties of Macrophages and CD8+ T Cells in the Tumor Microenvironment
Journal of Clinical and Translational Hepatology
7.5
Human umbilical cord mesenchymal stem cells ameliorate colon inflammation via modulation of gut microbiota-SCFAs-immune axis
Stem Cell Research & Therapy
4.8
MiR-9 promotes G-MDSC recruitment and tumor proliferation by targeting SOCS3 in breast cancer
FASEB JOURNAL
29
Hepatocyte CHRNA4 mediates the MASH-promotive effects of immune cell-produced acetylcholine and smoking exposure in mice and humans
Cell Metabolism
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Osteoarthrosis & Rheumatoid Arthritis
Sepsis
Systemic Lupus Erythematosus
Transplantation

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