Mouse CXCL2/MIP-2 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CXCL2
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	5.23 pg/mL
Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	81% – 99%
Average Recovery	0.92

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	5.23 pg/mL
Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	81% – 99%
Average Recovery	0.92
Intra Precision	1.2% – 6.8%
Inter-Precision	2.1% – 6.0%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL2/MIP-2 monoclonal antibody Mouse CXCL2/MIP-2 freeze-dried standard CXCL2/MIP-2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse MIP-2单克隆 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MIP-2 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MIP-2 in the samples.加入终止液终止反应，在450nm波长(参考波长570 – 630nm)测定吸光度值。

Targets

CXCL2

CXCL2 Target Infomation Overview

Target Symbol: CXCL2, C-X-C motif chemokine ligand 2
Gene Groups: Chemokine ligands
Alias: SCYB2; GROb; MIP-2a; MGSA-b; CINC-2a
Previous Names: GRO2
Alias Names: GRO2 oncogene; chemokine (C-X-C motif) ligand 2

CXCL2, C-X-C motif chemokine ligand 2 Target Infomation by Species

HumanMouseRat

Human CXCL2 Target Information

Target Symbol: CXCL2, C-X-C motif chemokine ligand 2
Alias:
- C-X-C motif chemokine 2
- chemokine (C-X-C motif) ligand 2
- CINC-2a
- gro-beta
- GRO2
- GRO2 oncogene
- GROb
- growth-regulated protein beta
- macrophage inflammatory protein 2-alpha
- melanoma growth stimulatory activity beta
- MGSA beta
- MGSA-b
- MIP-2a
- MIP2
- MIP2-alpha
- MIP2A
- SCYB2
NCBI_Gene: 2920
UniProtKB: P19875

Human CXCL2 Predicted Functions

Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in antimicrobial humoral immune response mediated by antimicrobial peptide; killing of cells of other organism; and response to molecule of bacterial origin. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Biomarker of COVID-19 and cystic fibrosis.

Mouse Cxcl2 Target Information

Target Symbol: Cxcl2, chemokine (C-X-C motif) ligand 2
Alias:
- CINC-2a
- Gro2
- GROb
- macrophage inflammatory protein 2
- Mgsa-b
- MIP-2
- MIP-2a
- Mip2
- Scyb
- Scyb2
- small inducible cytokine B subfamily
- small inducible cytokine subfamily, member 2
NCBI_Gene: 20310

Mouse Cxcl2 Predicted Functions

Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in response to molecule of bacterial origin. Located in extracellular space. Is expressed in several structures, including alimentary system; embryo mesenchyme; floor plate; respiratory system; and skin. Orthologous to several human genes including CXCL3 (C-X-C motif chemokine ligand 3).

Rat Cxcl2 Target Information

Target Symbol: Cxcl2, C-X-C motif chemokine ligand 2
Alias:
- C-X-C motif chemokine 2
- chemokine (C-X-C motif) ligand 2
- CINC-3
- cytokine-induced neutrophil chemoattractant 3
- macrophage inflammatory protein 2
- Mip-2
- MIP2
- Scyb2
- small inducible cytokine subfamily member 2
- small inducible cytokine subfamily, member 2
NCBI_Gene: 114105
UniProtKB: P30348

Rat Cxcl2 Predicted Functions

Enables chemokine activity. Involved in several processes, including cellular response to interleukin-1; cellular response to lipopolysaccharide; and neutrophil chemotaxis. Located in extracellular space. Biomarker of several diseases, including acute necrotizing pancreatitis; bile duct disease (multiple); brain edema; pleurisy; and pneumonia (multiple). Orthologous to human CXCL2 (C-X-C motif chemokine ligand 2).