EK2100
Mouse Growth Differentiation Factor 15 /GDF-15 ELISA Kit
$299.00 – $419.00
DATASHEET
Species | mouse |
---|---|
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | 100 μL (diluent) |
Sensitivity | 0.72 pg/mL |
Array Range | 7.81 - 500 pg/ml |
Assay Time | 3.5 h |
Recovery | 81.7% - 106.1% |
Average Recovery | 92% |
ELISA Kit Detail Information
Species | mouse |
---|---|
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | 100 μL (diluent) |
Sensitivity | 0.72 pg/mL |
Array Range | 7.81 - 500 pg/ml |
Assay Time | 3.5 h |
Recovery | 81.7% - 106.1% |
Average Recovery | 92% |
Intra Precision | 7.1% - 8.9% |
Inter Precision | 3.9% - 8.1% |
Plate | Detachable 96-well plate |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | GDF-15 Microplate GDF-15 Standard GDF-15 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer Substrate Stop Solution Washing Buffer Plate Covers |
Assay Principle | This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-mouse GDF-15 antibodies are precoated on a high-affinity ELISA plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the plate and incubated, allowing the GDF-15 present in the samples to bind to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, HRP-labeled streptavidin is added. After washing again, a chromogenic substrate TMB is added for color development under light protection. The intensity of the color reaction is directly proportional to the concentration of GDF-15 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
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