Name: Mouse Growth Differentiation Factor 15 /GDF-15 ELISA Kit
SKU: EK2100
Availability: InStock

EK2100

Mouse Growth Differentiation Factor 15 /GDF-15 ELISA Kit

$245.00 – $350.00

Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	0.72 pg/mL
Array Range	7.81 - 500 pg/ml
Assay Time	3.5 h
Recovery	81.7% - 106.1%
Average Recovery	92%

Size

Clear

ELISA Kit Detail Information

Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	0.72 pg/mL
Array Range	7.81 - 500 pg/ml
Assay Time	3.5 h
Recovery	81.7% - 106.1%
Average Recovery	92%
Intra Precision	7.1% - 8.9%
Inter Precision	3.9% - 8.1%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	GDF-15 Microplate GDF-15 Standard GDF-15 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer Substrate Stop Solution Washing Buffer Plate Covers
Assay Principle	This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-mouse GDF-15 antibodies are precoated on a high-affinity ELISA plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the plate and incubated, allowing the GDF-15 present in the samples to bind to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, HRP-labeled streptavidin is added. After washing again, a chromogenic substrate TMB is added for color development under light protection. The intensity of the color reaction is directly proportional to the concentration of GDF-15 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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