Mouse IL-22 ELISA Kit

$350.00$450.00

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TARGETSIL22
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.74 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery85% – 118%
Average Recovery0.94
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.74 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery85% – 118%
Average Recovery0.94
Intra Precision2.9% – 7.9%
Inter-Precision3.1% – 5.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-22 monoclonal antibody
Mouse IL-22 freeze-dried standard
IL-22 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-22 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-22 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-22 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL22

IL22 Target Infomation Overview

  • Target Symbol: IL22, interleukin 22
  • Gene Groups: Interleukins
  • Alias: ILTIF; IL-21; zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; IL-22; MGC79382; MGC79384
  • Alias Names: IL-10-related T-cell-derived inducible factor

IL22, interleukin 22 Target Infomation by Species

HumanMouseRat

Human IL22 Target Information

  • Target Symbol: IL22, interleukin 22
  • Alias:
    • cytokine Zcyto18
    • IL-10-related T-cell-derived inducible factor
    • IL-10-related T-cell-derived-inducible factor
    • IL-21
    • IL-22
    • IL-D110
    • IL-TIF
    • IL21
    • ILTIF
    • interleukin 21
    • interleukin-22
    • MGC79382
    • MGC79384
    • TIFa
    • TIFIL-23
    • zcyto18
  • NCBI_Gene: 50616
  • UniProtKB: Q9GZX6

Human IL22 Predicted Functions

Predicted to enable cytokine activity. Predicted to be involved in response to glucocorticoid and signal transduction. Predicted to be located in extracellular region. Biomarker of asthma; rhinitis; and tuberculosis.

Mouse Il22 Target Information

Mouse Il22 Predicted Functions

Predicted to enable cytokine activity. Acts upstream of or within several processes, including negative regulation of inflammatory response; reactive oxygen species metabolic process; and regulation of tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular space. Is expressed in retina and skin. Used to study liposarcoma. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL22 (interleukin 22).

Rat Il22 Target Information

Rat Il22 Predicted Functions

Involved in response to glucocorticoid. Located in extracellular space. Used to study asthma and myocarditis. Orthologous to human IL22 (interleukin 22).

IL22 Target News

    3.32
    ILC2 regulates hyperoxia-induced lung injury via an enhanced Th17 cell response in the BPD mouse model
    BMC Pulmonary Medicine
    10.8
    Disease-specific corona mediated co-delivery of MTX and siRNA-TNFα by a polypeptide nanoplatform with antigen-scavenging functions in psoriasis
    JOURNAL OF CONTROLLED RELEASE
    5.8
    Discovery of novel carboxylesterase 2 inhibitors for the treatment of delayed diarrhea and ulcerative colitis
    BIOCHEMICAL PHARMACOLOGY
    9.9
    Glucan from Dictyophora rubrovolvata boosts immunity by regulating AKT-STAT1 pathway to induce dendritic cells maturation and T cells differentiation
    Food Frontiers
    Allergic Reaction
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Systemic Lupus Erythematosus

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