EK2176

Mouse MCPT-1/mMCP-1 ELISA Kit

$245.00$350.00

Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity44.56 pg/mL
Array Range62.5 ng/mL – 4000 ng/mL
Assay Time3.5 h
Recovery80% – 89%
Average Recovery84%

ELISA Kit Detail Information

Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity44.56 pg/mL
Array Range62.5 ng/mL – 4000 ng/mL
Assay Time3.5 h
Recovery80% – 89%
Average Recovery84%
Intra Precision2.9% – 3.9%
Inter Precision1.4% – 3.5%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MCPT-1/mMCP-1 monoclonal antibody
Mouse MCPT-1/mMCP-1 freeze-dried standard
MCPT-1/mMCP-1 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse MCPT-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MCPT-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MCPT-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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