Mouse PDGF-BB ELISA Kit

$350.00$450.00

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TARGETSPDGFB
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity1.05 pg/mL
Range23.44 pg/mL – 1500 pg/mL
Assay Time3.5 h
Recovery92% – 122%
Average Recovery1.08
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity1.05 pg/mL
Range23.44 pg/mL – 1500 pg/mL
Assay Time3.5 h
Recovery92% – 122%
Average Recovery1.08
Intra Precision2.5% – 5.0%
Inter-Precision3.3%-5.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PDGF-BB monoclonal antibody
Mouse PDGF-BB freeze-dried standard
Mouse PDGF-BB detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse PDGF-BB antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, PDGF-BB present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PDGF-BB in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

PDGFB

PDGFB Target Infomation Overview

  • Target Symbol: PDGFB, platelet derived growth factor subunit B
  • Alias: SSV
  • Previous Names: SIS
  • Alias Names: oncogene SIS; becaplermin; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor beta polypeptide

PDGFB, platelet derived growth factor subunit B Target Infomation by Species

HumanMouseRat

Human PDGFB Target Information

  • Target Symbol: PDGFB, platelet derived growth factor subunit B
  • Alias:
    • becaplermin
    • c-sis
    • FLJ12858
    • IBGC5
    • PDGF subunit B
    • PDGF-2
    • PDGF, B chain
    • PDGF2
    • platelet-derived growth factor 2
    • platelet-derived growth factor B chain
    • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
    • platelet-derived growth factor subunit B
    • platelet-derived growth factor, B chain
    • Platelet-derived growth factor, beta polypeptide (oncogene SIS)
    • proto-oncogene c-Sis
    • SIS
    • SSV
  • NCBI_Gene: 5155
  • UniProtKB: P01127

Human PDGFB Predicted Functions

Enables several functions, including platelet-derived growth factor binding activity; protein dimerization activity; and receptor ligand activity. Involved in several processes, including positive regulation of cell migration; positive regulation of cell population proliferation; and positive regulation of phosphorylation. Located in cell surface. Implicated in basal ganglia calcification; clear cell renal cell carcinoma; connective tissue cancer (multiple); familial meningioma; and prostatic hypertrophy. Biomarker of several diseases, including Kuhnt-Junius degeneration; bone disease (multiple); clear cell renal cell carcinoma; diabetic retinopathy (multiple); and reproductive organ cancer (multiple).

Mouse Pdgfb Target Information

Mouse Pdgfb Predicted Functions

Predicted to enable several functions, including platelet-derived growth factor binding activity; protein dimerization activity; and receptor ligand activity. Involved in several processes, including metanephric glomerular mesangium development; paracrine signaling; and positive regulation of glomerular filtration. Acts upstream of or within several processes, including animal organ development; nervous system development; and regulation of cell migration. Predicted to be located in several cellular components, including basolateral plasma membrane; dendrite; and neuronal cell body. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; cardiovascular system; extraembryonic component; female reproductive system; and nervous system. Human ortholog(s) of this gene implicated in basal ganglia calcification; clear cell renal cell carcinoma; connective tissue cancer (multiple); familial meningioma; and prostatic hypertrophy. Orthologous to human PDGFB (platelet derived growth factor subunit B).

Rat Pdgfb Target Information

  • Target Symbol: Pdgfb, platelet derived growth factor subunit B
  • Alias:
    • c-sis
    • pdgf protein
    • PDGF subunit B
    • PDGF-2
    • platelet derived growth factor-bb
    • platelet derived growth factor, B polypeptide
    • Platelet-derived growth factor
    • platelet-derived growth factor B chain
    • platelet-derived growth factor beta polypeptide
    • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
    • Platelet-derived growth factor same as Sis (Simian sarcoma viral oncogene homologue c-sis)
    • platelet-derived growth factor subunit B
    • Platelet-derived growth factor, same as Sis (Simian sarcoma viral oncogene homologue, c-sis)
    • SIS
  • NCBI_Gene: 24628

Rat Pdgfb Predicted Functions

Enables signaling receptor binding activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of cell migration; and response to peptide hormone. Located in several cellular components, including basolateral plasma membrane; dendrite; and neuronal cell body. Used to study asthma; brain infarction; glomerulonephritis; and visual epilepsy. Biomarker of cholestasis; hypertension (multiple); liver cirrhosis; oligohydramnios; and ureteral obstruction. Human ortholog(s) of this gene implicated in basal ganglia calcification; clear cell renal cell carcinoma; connective tissue cancer (multiple); familial meningioma; and prostatic hypertrophy. Orthologous to human PDGFB (platelet derived growth factor subunit B).

PDGFB Target News

    4.9
    Salvianolic acid B promotes angiogenesis and inhibits cardiomyocyte apoptosis by regulating autophagy in myocardial ischemia
    Chinese Medicine
    5.4
    Icariside Ⅱ attenuates bleomycin-induced pulmonary fibrosis by modulating macrophage polarization
    JOURNAL OF ETHNOPHARMACOLOGY
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)

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