Mouse/Rat IGF-1 ELISA Kit

$350.00$450.00

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TARGETSIGF1
SPECIESMouse Rat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.82 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery95% – 110%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.82 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery95% – 110%
Average Recovery1.02
Intra Precision2.9% – 3.6%
Inter-Precision2.0% – 4.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGF-1 monoclonal antibody
Mouse /Rat IGF-1 freeze-dried standard
IGF-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse /rat IGF-1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IGF-1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGF-1 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IGF1

IGF1 Target Infomation Overview

  • Target Symbol: IGF1, insulin like growth factor 1
  • Gene Groups: Neuropeptides
  • Alias: IGF1A; IGFI; IGF-I; IGF
  • Alias Names: somatomedin C; insulin-like growth factor 1 (somatomedin C)

IGF1, insulin like growth factor 1 Target Infomation by Species

HumanMouseRat

Human IGF1 Target Information

  • Target Symbol: IGF1, insulin like growth factor 1
  • Alias:
    • IGF
    • IGF-I
    • IGF-IA
    • IGF-IB
    • IGF1A
    • IGFI
    • insulin-like growth factor 1
    • insulin-like growth factor 1 (somatomedin C)
    • insulin-like growth factor I
    • insulin-like growth factor IA
    • insulin-like growth factor IB
    • mechano growth factor
    • MGF
    • somatomedin-C
  • NCBI_Gene: 3479
  • UniProtKB: P05019

Human IGF1 Predicted Functions

Enables signaling receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within cell activation. Located in extracellular space. Part of alphav-beta3 integrin-IGF-1-IGF1R complex and insulin-like growth factor ternary complex. Implicated in several diseases, including acne; brain ischemia; diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Biomarker of several diseases, including Henoch-Schoenlein purpura; artery disease (multiple); autoimmune disease (multiple); bone disease (multiple); and eye disease (multiple).

Mouse Igf1 Target Information

Mouse Igf1 Predicted Functions

Enables insulin receptor binding activity and insulin-like growth factor receptor binding activity. Involved in negative regulation of gene expression; negative regulation of release of cytochrome c from mitochondria; and positive regulation of myelination. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and regulation of signal transduction. Located in exocytic vesicle and extracellular space. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including acne; artery disease (multiple); diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Orthologous to human IGF1 (insulin like growth factor 1).

Rat Igf1 Target Information

Rat Igf1 Predicted Functions

Enables protein serine/threonine kinase activator activity; signaling receptor binding activity; and steroid binding activity. Involved in several processes, including cellular response to hormone stimulus; negative regulation of cell death; and positive regulation of cell differentiation. Located in several cellular components, including interstitial matrix; neuronal cell body; and platelet alpha granule. Used to study brain ischemia; diabetic neuropathy; and hypertension. Biomarker of several diseases, including alcoholic neuropathy; artery disease (multiple); congenital diaphragmatic hernia; folic acid deficiency anemia; and hypothyroidism (multiple). Human ortholog(s) of this gene implicated in several diseases, including acne; brain ischemia; diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Orthologous to human IGF1 (insulin like growth factor 1).

IGF1 Target News

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    JOURNAL OF CONTROLLED RELEASE
    16.744
    Cartilage-targeting mRNA-lipid nanoparticles rescue perifocal apoptotic chondrocytes for integrative cartilage repair
    CHEMICAL ENGINEERING JOURNAL
    15.1
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    Advanced Science
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Tumor Marker

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