Mouse/Rat IGF-1 ELISA Kit

$350.00$450.00

TARGETSIGF1
SPECIESMouse Rat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.82 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery95% – 110%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity0.82 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery95% – 110%
Average Recovery1.02
Intra Precision2.9% – 3.6%
Inter-Precision2.0% – 4.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGF-1 monoclonal antibody
Mouse /Rat IGF-1 freeze-dried standard
IGF-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse /rat IGF-1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IGF-1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGF-1 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IGF1

IGF1 Target Infomation Overview

  • Target Symbol: IGF1, insulin like growth factor 1
  • Gene Groups: Neuropeptides
  • Alias: IGF1A; IGFI; IGF-I; IGF
  • Alias Names: somatomedin C; insulin-like growth factor 1 (somatomedin C)

IGF1, insulin like growth factor 1 Target Infomation by Species

HumanMouseRat

Human IGF1 Target Information

  • Target Symbol: IGF1, insulin like growth factor 1
  • Alias:
    • IGF
    • IGF-I
    • IGF-IA
    • IGF-IB
    • IGF1A
    • IGFI
    • insulin-like growth factor 1
    • insulin-like growth factor 1 (somatomedin C)
    • insulin-like growth factor I
    • insulin-like growth factor IA
    • insulin-like growth factor IB
    • mechano growth factor
    • MGF
    • somatomedin-C
  • NCBI_Gene: 3479
  • UniProtKB: P05019

Human IGF1 Predicted Functions

Enables signaling receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within cell activation. Located in extracellular space. Part of alphav-beta3 integrin-IGF-1-IGF1R complex and insulin-like growth factor ternary complex. Implicated in several diseases, including acne; brain ischemia; diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Biomarker of several diseases, including Henoch-Schoenlein purpura; artery disease (multiple); autoimmune disease (multiple); bone disease (multiple); and eye disease (multiple).

Mouse Igf1 Target Information

Mouse Igf1 Predicted Functions

Enables insulin receptor binding activity and insulin-like growth factor receptor binding activity. Involved in negative regulation of gene expression; negative regulation of release of cytochrome c from mitochondria; and positive regulation of myelination. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and regulation of signal transduction. Located in exocytic vesicle and extracellular space. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including acne; artery disease (multiple); diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Orthologous to human IGF1 (insulin like growth factor 1).

Rat Igf1 Target Information

Rat Igf1 Predicted Functions

Enables protein serine/threonine kinase activator activity; signaling receptor binding activity; and steroid binding activity. Involved in several processes, including cellular response to hormone stimulus; negative regulation of cell death; and positive regulation of cell differentiation. Located in several cellular components, including interstitial matrix; neuronal cell body; and platelet alpha granule. Used to study brain ischemia; diabetic neuropathy; and hypertension. Biomarker of several diseases, including alcoholic neuropathy; artery disease (multiple); congenital diaphragmatic hernia; folic acid deficiency anemia; and hypothyroidism (multiple). Human ortholog(s) of this gene implicated in several diseases, including acne; brain ischemia; diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Orthologous to human IGF1 (insulin like growth factor 1).

IGF1 Target News

[catlist tags=”igf1″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

11.467
Insulin-like growth factor binding protein 4 loaded electrospun membrane ameliorating tendon injury by promoting retention of IGF-1
JOURNAL OF CONTROLLED RELEASE
16.744
Cartilage-targeting mRNA-lipid nanoparticles rescue perifocal apoptotic chondrocytes for integrative cartilage repair
CHEMICAL ENGINEERING JOURNAL
15.1
Regeneration of Thyroid Glands in the Spleen Restores Homeostasis in Thyroidectomy Mice
Advanced Science
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Tumor Marker

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