Mouse/Rat IGF-1 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	IGF1
SPECIES	Mouse Rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.82 pg/mL
Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	95% – 110%
Average Recovery	1.02

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	0.82 pg/mL
Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	95% – 110%
Average Recovery	1.02
Intra Precision	2.9% – 3.6%
Inter-Precision	2.0% – 4.1%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGF-1 monoclonal antibody Mouse /Rat IGF-1 freeze-dried standard IGF-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse /rat IGF-1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IGF-1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGF-1 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IGF1

IGF1 Target Infomation Overview

Target Symbol: IGF1, insulin like growth factor 1
Gene Groups: Neuropeptides
Alias: IGF1A; IGFI; IGF-I; IGF
Alias Names: somatomedin C; insulin-like growth factor 1 (somatomedin C)

IGF1, insulin like growth factor 1 Target Infomation by Species

HumanMouseRat

Human IGF1 Target Information

Target Symbol: IGF1, insulin like growth factor 1
Alias:
- IGF
- IGF-I
- IGF-IA
- IGF-IB
- IGF1A
- IGFI
- insulin-like growth factor 1
- insulin-like growth factor 1 (somatomedin C)
- insulin-like growth factor I
- insulin-like growth factor IA
- insulin-like growth factor IB
- mechano growth factor
- MGF
- somatomedin-C
NCBI_Gene: 3479
UniProtKB: P05019

Human IGF1 Predicted Functions

Enables signaling receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within cell activation. Located in extracellular space. Part of alphav-beta3 integrin-IGF-1-IGF1R complex and insulin-like growth factor ternary complex. Implicated in several diseases, including acne; brain ischemia; diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Biomarker of several diseases, including Henoch-Schoenlein purpura; artery disease (multiple); autoimmune disease (multiple); bone disease (multiple); and eye disease (multiple).

Mouse Igf1 Target Information

Target Symbol: Igf1, insulin-like growth factor 1
Alias:
- C730016P09Rik
- Igf-1
- Igf-I
- RIKEN cDNA C730016P09 gene
NCBI_Gene: 16000

Mouse Igf1 Predicted Functions

Enables insulin receptor binding activity and insulin-like growth factor receptor binding activity. Involved in negative regulation of gene expression; negative regulation of release of cytochrome c from mitochondria; and positive regulation of myelination. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and regulation of signal transduction. Located in exocytic vesicle and extracellular space. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including acne; artery disease (multiple); diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Orthologous to human IGF1 (insulin like growth factor 1).

Rat Igf1 Target Information

Target Symbol: Igf1, insulin-like growth factor 1
Alias:
- IGF
- IGF-I
- insulin-like growth factor I
- somatomedin
NCBI_Gene: 24482

Rat Igf1 Predicted Functions

Enables protein serine/threonine kinase activator activity; signaling receptor binding activity; and steroid binding activity. Involved in several processes, including cellular response to hormone stimulus; negative regulation of cell death; and positive regulation of cell differentiation. Located in several cellular components, including interstitial matrix; neuronal cell body; and platelet alpha granule. Used to study brain ischemia; diabetic neuropathy; and hypertension. Biomarker of several diseases, including alcoholic neuropathy; artery disease (multiple); congenital diaphragmatic hernia; folic acid deficiency anemia; and hypothyroidism (multiple). Human ortholog(s) of this gene implicated in several diseases, including acne; brain ischemia; diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Orthologous to human IGF1 (insulin like growth factor 1).