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Mouse Total MMP-3 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL(diluent) |
| Sensitivity | 15.99 pg/mL |
| Range | 46.88 pg/mL – 3000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 75% – 95% |
| Average Recovery | 0.88 |
| Intra Precision | 2.9% – 3.6% |
| Inter-Precision | 3.4%-4.8% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-total MMP-3 monoclonal antibody Mouse total MMP-3 freeze-dried standard Mouse total MMP-3 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse total MMP-3 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, total MMP-3 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of total MMP-3 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
MMP3
MMP3 Target Infomation Overview
- Target Symbol: MMP3, matrix metallopeptidase 3
- Gene Groups: M10 matrix metallopeptidases
- Previous Names: STMY1; STMY
- Alias Names: matrix metalloproteinase 3 (stromelysin 1, progelatinase); stromelysin 1
MMP3, matrix metallopeptidase 3 Target Infomation by Species
HumanMouseRat
Human MMP3 Target Information
- Target Symbol: MMP3, matrix metallopeptidase 3
- Alias:
- CHDS6
- matrix metallopeptidase 3 (stromelysin 1, progelatinase)
- matrix metalloproteinase 3 (stromelysin 1, progelatinase)
- matrix metalloproteinase-3
- MGC126102
- MGC126103
- MGC126104
- MMP-3
- proteoglycanase
- SL-1
- STMY
- STMY1
- STR1
- stromelysin-1
- transin-1
- NCBI_Gene: 4314
- UniProtKB: P08254
Human MMP3 Predicted Functions
Enables endopeptidase activity and metallopeptidase activity. Involved in several processes, including cellular response to UV-A; negative regulation of hydrogen peroxide metabolic process; and positive regulation of protein-containing complex assembly. Predicted to be located in cytosol and mitochondrion. Implicated in several diseases, including artery disease (multiple); carcinoma (multiple); end stage renal disease; osteoarthritis; and von Hippel-Lindau disease. Biomarker of several diseases, including Takayasu’s arteritis; arthritis (multiple); autoimmune disease (multiple); conjunctivochalasis; and lung disease (multiple).
Mouse Mmp3 Target Information
- Target Symbol: Mmp3, matrix metallopeptidase 3
- Alias:
- progelatinase
- SLN-1
- SLN1
- Stmy1
- STR-1
- Str1
- stromelysin 1
- stromelysin-1
- NCBI_Gene: 17392
Mouse Mmp3 Predicted Functions
Predicted to enable metalloendopeptidase activity. Involved in several processes, including negative regulation of protein kinase B signaling; positive regulation of oxidative stress-induced cell death; and protein catabolic process. Acts upstream of or within cellular response to amino acid stimulus and regulation of cell migration. Located in cytosol and mitochondrion. Is expressed in several structures, including axial skeleton thoracic region; genitourinary system; liver; oral region epithelium; and palatal shelf. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); carcinoma (multiple); end stage renal disease; osteoarthritis; and von Hippel-Lindau disease. Orthologous to human MMP3 (matrix metallopeptidase 3).
Rat Mmp3 Target Information
- Target Symbol: Mmp3, matrix metallopeptidase 3
- Alias:
- matrix metalloproteinase 3
- matrix metalloproteinase-3
- MMP-3
- PTR1 protein
- SL-1
- stromelysin-1
- transin-1
- NCBI_Gene: 171045
Rat Mmp3 Predicted Functions
Predicted to enable metalloendopeptidase activity. Involved in several processes, including cellular response to cell-matrix adhesion; cellular response to interleukin-1; and response to estradiol. Located in several cellular components, including cell body; dendrite; and extracellular space. Part of protein-containing complex. Biomarker of several diseases, including abdominal aortic aneurysm; degenerative disc disease; gastric ulcer; glomerulonephritis (multiple); and primary biliary cholangitis. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); carcinoma (multiple); end stage renal disease; osteoarthritis; and von Hippel-Lindau disease. Orthologous to human MMP3 (matrix metallopeptidase 3).
