Mouse VEGF ELISA Kit

$350.00$450.00

TARGETS VEGFA
SPECIES Mouse
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume Serum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity 1.97 pg/mL
Range 31.25 pg/mL – 2000 pg/mL
Assay Time 3.5 h
Recovery 89% – 110%
Average Recovery 0.98
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume Serum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity 1.97 pg/mL
Range 31.25 pg/mL – 2000 pg/mL
Assay Time 3.5 h
Recovery 89% – 110%
Average Recovery 0.98
Intra Precision 3.8% – 7.6%
Inter-Precision 2.3% – 5.2%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-VEGF monoclonal antibody
Mouse VEGF freeze-dried standard
VEGF detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse VEGF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, VEGF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of VEGF in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

VEGFA

VEGFA Target Infomation Overview

  • Target Symbol: VEGFA, vascular endothelial growth factor A
  • Gene Groups: VEGF family
  • Alias: VEGF-A; VPF
  • Previous Names: VEGF
  • Alias Names: vascular endothelial growth factor

VEGFA, vascular endothelial growth factor A Target Infomation by Species

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Human VEGFA Target Information

  • Target Symbol: VEGFA, vascular endothelial growth factor A
  • Alias:
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • vascular endothelial growth factor A121
    • vascular endothelial growth factor A165
    • vascular permeability factor
    • VEGF
    • VEGF-A
    • VPF
  • NCBI_Gene: 7422
  • UniProtKB: P15692

Human VEGFA Predicted Functions

Enables several functions, including fibronectin binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell migration; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including branching involved in blood vessel morphogenesis; positive regulation of endothelial cell proliferation; and regulation of nitric oxide mediated signal transduction. Located in several cellular components, including adherens junction; cell surface; and secretory granule. Implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); inflammatory bowel disease (multiple); and respiratory system cancer (multiple).

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Mouse Vegfa Target Information

Mouse Vegfa Predicted Functions

Enables extracellular matrix binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including generation of neurons; regulation of signal transduction; and vasculature development. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and vasculature development. Located in cytoplasm; extracellular space; and nucleus. Is expressed in several structures, including alimentary system; brain; egg cylinder; genitourinary system; and heart. Used to study DiGeorge syndrome; amyotrophic lateral sclerosis type 1; newborn respiratory distress syndrome; and psoriasis. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).

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Rat Vegfa Target Information

Rat Vegfa Predicted Functions

Enables several functions, including identical protein binding activity; receptor ligand activity; and vascular endothelial growth factor receptor 2 binding activity. Involved in several processes, including negative regulation of cell death; response to steroid hormone; and response to vitamin. Located in basement membrane; extracellular space; and vesicle. Used to study several diseases, including brain edema; limb ischemia; middle cerebral artery infarction; ovarian hyperstimulation syndrome; and proliferative diabetic retinopathy. Biomarker of several diseases, including adhesions of uterus; attention deficit hyperactivity disorder; brain edema; liver disease (multiple); and retinal vascular disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); gastrointestinal system cancer (multiple); hematologic cancer (multiple); hypertension (multiple); and reproductive organ cancer (multiple). Orthologous to human VEGFA (vascular endothelial growth factor A).

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VEGFA Target News

[catlist tags=”vegfa” template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

8.5
Natural Flavonoid-Derived Enzyme Mimics DHKNase Balance the Two-Edged Reactive Oxygen Species Function for Wound Healing and Inflammatory Bowel Disease Therapy.
Research
7.5
Engineering an energy-dissipating hybrid tissue in vivo for obesity treatment
Cell Reports
3.3
Effect of M2-macrophage treated lymphatic endothelial cells on angiogenesis that promoted osteointegration
EXPERIMENTAL CELL RESEARCH
5.3
All-in-one hydrogel patches with sprayed bFGF-loaded GelMA microspheres for infected wound healing studies
INTERNATIONAL JOURNAL OF PHARMACEUTICS
8.1
TMEM9 promotes lung adenocarcinoma progression via activating the MEK/ERK/STAT3 pathway to induce VEGF expression
Cell Death & Disease
13.3
Hamburger-like biomimetic nutrient periosteum with osteoimmunomodulation, angio-/osteo-genesis capacity promoted critical-size bone defect repair
CHEMICAL ENGINEERING JOURNAL
10.5
Brucea javanica derived exosome-like nanovesicles deliver miRNAs for cancer therapy
JOURNAL OF CONTROLLED RELEASE
8.3
Hollow Copper Sulfide Photothermal Nanodelivery Platform Boosts Angiogenesis of Diabetic Wound by Scavenging Reactive Oxygen Species
ACS Applied Materials & Interfaces
10.5
Blocking tumor-platelet crosstalk to prevent tumor metastasis via reprograming glycolysis using biomimetic membrane-hybridized liposomes
JOURNAL OF CONTROLLED RELEASE
7.5
Umbilical cord-derived mesenchymal stem cells cultured in the MCL medium for aplastic anemia therapy
Stem Cell Research & Therapy
8
Co-Delivery Nanomicelles for Potentiating TNBC Immunotherapy by Synergetically Reshaping CAFs-Mediated Tumor Stroma and Reprogramming Immunosuppressive Microenvironment
International Journal of Nanomedicine
4.9
Salvianolic acid B promotes angiogenesis and inhibits cardiomyocyte apoptosis by regulating autophagy in myocardial ischemia
Chinese Medicine
5.7
Reprogramming tumor microenvironment via dual targeting co-delivery of regorafenib and alpha-difluoromethylornithine in osteosarcoma
Cancer Nanotechnology
11.5
Anticoagulants Enhance Molecular and Cellular Immunotherapy of Cancer by Improving Tumor Microcirculation Structure and Function and Redistributing Tumor Infiltrates
CLINICAL CANCER RESEARCH
2.3
The histologic reaction and permanence of hyaluronic acid gel, calcium hydroxylapatite microspheres, and extracellular matrix bio gel
Journal of Cosmetic Dermatology
8.5
Endothelial Stat3 activation promotes osteoarthritis development
CELL PROLIFERATION
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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