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Prostaglandin E2/PGE2 Competitive ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL(diluent) |
| Sensitivity | 1.08 pg/mL |
| Range | 15.63 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 92 – 102% |
| Average Recovery | 0.99 |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL(diluent) |
| Sensitivity | 1.08 pg/mL |
| Range | 15.63 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 92 – 102% |
| Average Recovery | 0.99 |
| Intra Precision | 3.9% – 4.3% |
| Inter-Precision | 5.2% – 6.5% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Mouse IgG的96孔聚苯乙烯酶标板 PGE2 freeze-dried standard PGE2-biotin偶联物冻干粉 PGE2抗体冻干粉 HRP-labeled streptavidin冻干粉 Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes a solid-phase competitive enzyme-linked immunosorbent assay (ELISA) technique. Rabbit anti-mouse antibodies are precoated on a high-affinity ELISA plate. Specific monoclonal antibodies against PGE2 are added to the wells and incubated to bind to the immobilized antibodies. After washing, biotinylated PGE2 and unlabeled PGE2 or samples are added, competing for limited binding sites on the monoclonal antibodies. After washing to remove unbound substances, HRP-labeled streptavidin is added. After washing again, a chromogenic substrate is added for color development, and the intensity of the color reaction is inversely proportional to the concentration of PGE2 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). This assay kit has been validated for human, mouse, and rat serum samples, but it is expected to detect PGE2 from other mammalian species as well. |
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