Rat TIMP-1 ELISA Kit

$350.00$450.00

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TARGETSTIMP1
SPECIESRat
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity3.66 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery88% – 114%
Average Recovery1.01
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity3.66 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery88% – 114%
Average Recovery1.01
Intra Precision3.8% – 4.4%
Inter-Precision3.4%-4.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TIMP-1 monoclonal antibody
Rat TIMP-1 freeze-dried standard
Rat TIMP-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat TIMP-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TIMP-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TIMP-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TIMP1

TIMP1 Target Infomation Overview

  • Target Symbol: TIMP1, TIMP metallopeptidase inhibitor 1
  • Gene Groups: Tissue inhibitor of metallopeptidases
  • Alias: EPO
  • Previous Names: TIMP; CLGI
  • Alias Names: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)

TIMP1, TIMP metallopeptidase inhibitor 1 Target Infomation by Species

HumanMouseRat

Human TIMP1 Target Information

  • Target Symbol: TIMP1, TIMP metallopeptidase inhibitor 1
  • Alias:
    • CLGI
    • collagenase inhibitor
    • EPA
    • EPO
    • erythroid potentiating activity
    • erythroid-potentiating activity
    • fibroblast collagenase inhibitor
    • FLJ90373
    • HCI
    • metalloproteinase inhibitor 1
    • TIMP
    • TIMP-1
    • tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
    • tissue inhibitor of metalloproteinases 1
  • NCBI_Gene: 7076
  • UniProtKB: P01033

Human TIMP1 Predicted Functions

Enables cytokine activity; metalloendopeptidase inhibitor activity; and zinc ion binding activity. Involved in several processes, including cellular response to UV-A; negative regulation of proteolysis; and negative regulation of trophoblast cell migration. Located in extracellular space. Biomarker of several diseases, including CAKUT2; Down syndrome; artery disease (multiple); carcinoma (multiple); and diabetes mellitus (multiple).

Mouse Timp1 Target Information

Mouse Timp1 Predicted Functions

Predicted to enable several functions, including cytokine activity; metalloendopeptidase inhibitor activity; and zinc ion binding activity. Acts upstream of or within connective tissue replacement involved in inflammatory response wound healing. Located in basement membrane. Is expressed in several structures, including early conceptus; hindlimb cartilage condensation; jaw; reproductive system; and skeletal system. Orthologous to human TIMP1 (TIMP metallopeptidase inhibitor 1).

Rat Timp1 Target Information

  • Target Symbol: Timp1, TIMP metallopeptidase inhibitor 1
  • Alias:
    • metalloproteinase inhibitor 1
    • Timp
    • TIMP-1
    • tissue inhibitor of metallopeptidase 1
    • tissue inhibitor of metalloproteinase 1
    • tissue inhibitor of metalloproteinases 1
  • NCBI_Gene: 116510
  • UniProtKB: P30120

Rat Timp1 Predicted Functions

Enables protease binding activity. Involved in several processes, including cartilage development; response to peptide hormone; and wound healing. Located in basement membrane. Used to study degenerative disc disease. Biomarker of cholestasis; gastric ulcer; heart disease (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human TIMP1 (TIMP metallopeptidase inhibitor 1).

TIMP1 Target News

    10.633
    Dual delivery of an NF-κB inhibitor and IL-10 through supramolecular hydrogels polarizes macrophages and promotes cardiac repair after myocardial infarction
    Acta Biomaterialia
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Tumor Marker

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