Product Category
Newest Products
Human 4-1BB/TNFRSF9/CD137 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 1.58 pg/mL |
| Range | 4.69 pg/mL – 300 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% – 117% |
| Average Recovery | 1.02 |
| Intra Precision | 4.7% – 5.0% |
| Inter-Precision | 3.2% – 6.6% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-4-1BB/TNFRSF9/CD137 monoclonal antibody Human 4-1BB/TNFRSF9/CD137 freeze-dried standard 4-1BB/TNFRSF9/CD137 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human 4-1BB antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, 4-1BB present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of 4-1BB in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
TNFRSF9
TNFRSF9 Target Infomation Overview
- Target Symbol: TNFRSF9, TNF receptor superfamily member 9
- Gene Groups: CD molecules; Tumor necrosis factor receptor superfamily
- Alias: CD137; 4-1BB
- Previous Names: ILA
- Alias Names: tumor necrosis factor receptor superfamily, member 9
TNFRSF9, TNF receptor superfamily member 9 Target Infomation by Species
HumanMouseRat
Human TNFRSF9 Target Information
- Target Symbol: TNFRSF9, TNF receptor superfamily member 9
- Alias:
- 4-1BB
- 4-1BB ligand receptor
- CD137
- CD137 antigen
- CDw137
- FLJ43501
- homolog of mouse 4-1BB
- ILA
- induced by lymphocyte activation (ILA)
- interleukin-activated receptor, homolog of mouse Ly63
- MGC2172
- receptor protein 4-1BB
- T cell antigen ILA
- T-cell antigen 4-1BB homolog
- T-cell antigen ILA
- tumor necrosis factor receptor superfamily member 9
- tumor necrosis factor receptor superfamily, member 9
- NCBI_Gene: 3604
- UniProtKB: Q07011
Human TNFRSF9 Predicted Functions
Predicted to enable cytokine binding activity; identical protein binding activity; and signaling receptor activity. Predicted to be involved in regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of interleukin-10 production and negative regulation of interleukin-12 production. Predicted to be located in extracellular space. Predicted to be active in external side of plasma membrane.
Mouse Tnfrsf9 Target Information
- Target Symbol: Tnfrsf9, tumor necrosis factor receptor superfamily, member 9
- Alias:
- 4-1BB
- A930040I11Rik
- AA408498
- AI325004
- CD antigen 137
- Cd137
- CDw137
- expressed sequence AA408498
- expressed sequence AI325004
- ILA
- Ly63
- lymphocyte antigen 63
- RIKEN cDNA A930040I11 gene
- NCBI_Gene: 21942
Mouse Tnfrsf9 Predicted Functions
Enables cytokine binding activity; identical protein binding activity; and signaling receptor activity. Acts upstream of or within negative regulation of interleukin-10 production; negative regulation of interleukin-12 production; and regulation of cell population proliferation. Located in external side of plasma membrane and extracellular space. Is expressed in renal vasculature. Orthologous to human TNFRSF9 (TNF receptor superfamily member 9).
Rat Tnfrsf9 Target Information
- Target Symbol: Tnfrsf9, TNF receptor superfamily member 9
- Alias:
- 4-1BB
- LOC500590
- MGC114552
- similar to T-cell antigen 4-1BB precursor – mouse
- tumor necrosis factor receptor superfamily member 9
- tumor necrosis factor receptor superfamily, member 9
- NCBI_Gene: 500590
- UniProtKB: Q4V895
Rat Tnfrsf9 Predicted Functions
Predicted to enable cytokine binding activity; identical protein binding activity; and signaling receptor activity. Predicted to be involved in regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of interleukin-10 production and negative regulation of interleukin-12 production. Predicted to be located in extracellular space and plasma membrane. Predicted to be active in external side of plasma membrane. Orthologous to human TNFRSF9 (TNF receptor superfamily member 9).
