Human 4-1BB/TNFRSF9/CD137 ELISA Kit

$350.00$450.00

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TARGETSTNFRSF9
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity1.58 pg/mL
Range4.69 pg/mL – 300 pg/mL
Assay Time3.5 h
Recovery85% – 117%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity1.58 pg/mL
Range4.69 pg/mL – 300 pg/mL
Assay Time3.5 h
Recovery85% – 117%
Average Recovery1.02
Intra Precision4.7% – 5.0%
Inter-Precision3.2% – 6.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-4-1BB/TNFRSF9/CD137 monoclonal antibody
Human 4-1BB/TNFRSF9/CD137 freeze-dried standard
4-1BB/TNFRSF9/CD137 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human 4-1BB antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, 4-1BB present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of 4-1BB in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TNFRSF9

TNFRSF9 Target Infomation Overview

  • Target Symbol: TNFRSF9, TNF receptor superfamily member 9
  • Gene Groups: CD molecules; Tumor necrosis factor receptor superfamily
  • Alias: CD137; 4-1BB
  • Previous Names: ILA
  • Alias Names: tumor necrosis factor receptor superfamily, member 9

TNFRSF9, TNF receptor superfamily member 9 Target Infomation by Species

HumanMouseRat

Human TNFRSF9 Target Information

  • Target Symbol: TNFRSF9, TNF receptor superfamily member 9
  • Alias:
    • 4-1BB
    • 4-1BB ligand receptor
    • CD137
    • CD137 antigen
    • CDw137
    • FLJ43501
    • homolog of mouse 4-1BB
    • ILA
    • induced by lymphocyte activation (ILA)
    • interleukin-activated receptor, homolog of mouse Ly63
    • MGC2172
    • receptor protein 4-1BB
    • T cell antigen ILA
    • T-cell antigen 4-1BB homolog
    • T-cell antigen ILA
    • tumor necrosis factor receptor superfamily member 9
    • tumor necrosis factor receptor superfamily, member 9
  • NCBI_Gene: 3604
  • UniProtKB: Q07011

Human TNFRSF9 Predicted Functions

Predicted to enable cytokine binding activity; identical protein binding activity; and signaling receptor activity. Predicted to be involved in regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of interleukin-10 production and negative regulation of interleukin-12 production. Predicted to be located in extracellular space. Predicted to be active in external side of plasma membrane.

Mouse Tnfrsf9 Target Information

Mouse Tnfrsf9 Predicted Functions

Enables cytokine binding activity; identical protein binding activity; and signaling receptor activity. Acts upstream of or within negative regulation of interleukin-10 production; negative regulation of interleukin-12 production; and regulation of cell population proliferation. Located in external side of plasma membrane and extracellular space. Is expressed in renal vasculature. Orthologous to human TNFRSF9 (TNF receptor superfamily member 9).

Rat Tnfrsf9 Target Information

  • Target Symbol: Tnfrsf9, TNF receptor superfamily member 9
  • Alias:
    • 4-1BB
    • LOC500590
    • MGC114552
    • similar to T-cell antigen 4-1BB precursor – mouse
    • tumor necrosis factor receptor superfamily member 9
    • tumor necrosis factor receptor superfamily, member 9
  • NCBI_Gene: 500590
  • UniProtKB: Q4V895

Rat Tnfrsf9 Predicted Functions

Predicted to enable cytokine binding activity; identical protein binding activity; and signaling receptor activity. Predicted to be involved in regulation of cell population proliferation. Predicted to act upstream of or within negative regulation of interleukin-10 production and negative regulation of interleukin-12 production. Predicted to be located in extracellular space and plasma membrane. Predicted to be active in external side of plasma membrane. Orthologous to human TNFRSF9 (TNF receptor superfamily member 9).

TNFRSF9 Target News

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