Human alpha-Galactosidase A/GLA ELISA Kit

$350.00$450.00

Download:
TARGETSGLA
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity53.55 pg/mL
Range0.5 ng/mL – 32 ng/mL
Assay Time3.5 h
Recovery73% – 127%
Average Recovery0.93
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity53.55 pg/mL
Range0.5 ng/mL – 32 ng/mL
Assay Time3.5 h
Recovery73% – 127%
Average Recovery0.93
Intra Precision4.9% – 8.5%
Inter-Precision3.5%-7.7%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-alpha-Galactosidase A/GLA monoclonal antibody
Human alpha-Galactosidase A/GLA freeze-dried standard
Human alpha-Galactosidase A/GLA detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-human GLA antibodies are pre-coated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, the GLA present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, detection antibodies labeled with horseradish peroxidase (HRP) are added and incubated. After washing to remove unbound detection antibodies, the TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is directly proportional to the concentration of GLA in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

GLA

GLA Target Infomation Overview

  • Target Symbol: GLA, galactosidase alpha
  • Gene Groups: Galactosidases alpha
  • Alias: GALA

GLA, galactosidase alpha Target Infomation by Species

HumanMouseRat

Human GLA Target Information

  • Target Symbol: GLA, galactosidase alpha
  • Alias:
    • agalsidase alfa
    • alpha-D-galactosidase A
    • alpha-D-galactoside galactohydrolase 1
    • alpha-gal A
    • alpha-galactosidase A
    • GALA
    • galactosidase, alpha
    • galactosylgalactosylglucosylceramidase GLA
    • melibiase
  • NCBI_Gene: 2717
  • UniProtKB: P06280

Human GLA Predicted Functions

Enables hydrolase activity, hydrolyzing O-glycosyl compounds; protein homodimerization activity; and signaling receptor binding activity. Involved in glycosphingolipid catabolic process and oligosaccharide metabolic process. Located in Golgi apparatus; extracellular region; and lysosome. Implicated in Fabry disease.

Mouse Gla Target Information

Mouse Gla Predicted Functions

Enables alpha-galactosidase activity. Involved in negative regulation of nitric oxide biosynthetic process and negative regulation of nitric-oxide synthase activity. Acts upstream of or within glycosylceramide catabolic process. Located in extracellular space and lysosome. Is expressed in several structures, including adrenal gland; alimentary system; brain; genitourinary system; and liver and biliary system. Used to study Fabry disease. Human ortholog(s) of this gene implicated in Fabry disease. Orthologous to human GLA (galactosidase alpha).

Rat Gla Target Information

Rat Gla Predicted Functions

Enables alpha-galactosidase activity and galactoside binding activity. Predicted to be involved in several processes, including glycosylceramide catabolic process; negative regulation of nitric oxide biosynthetic process; and negative regulation of nitric-oxide synthase activity. Located in lysosome. Used to study Fabry disease and lysosomal storage disease. Human ortholog(s) of this gene implicated in Fabry disease. Orthologous to human GLA (galactosidase alpha).

GLA Target News

    Related Products

    more +

    EK1392

    $350.00$450.00

    EK1341

    $350.00$450.00

    EK1C01

    $350.00$450.00

    Same Target