Human B4GALT1/GGTB2 ELISA Kit

$350.00$450.00

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TARGETSB4GALT1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.06 ng/mL
Range1.56 ng/mL – 100 ng/mL
Assay Time3.5 h
Recovery105% – 120%
Average Recovery1.15
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.06 ng/mL
Range1.56 ng/mL – 100 ng/mL
Assay Time3.5 h
Recovery105% – 120%
Average Recovery1.15
Intra Precision2.9% – 4.4%
Inter-Precision3.0% – 3.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-B4GALT1/GGTB2 monoclonal antibody
Human B4GALT1/GGTB2 freeze-dried standard
B4GALT1/GGTB2 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human B4GALT1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, B4GALT1 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of B4GALT1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

B4GALT1

B4GALT1 Target Infomation Overview

  • Target Symbol: B4GALT1, beta-1,4-galactosyltransferase 1
  • Gene Groups: Beta 4-glycosyltransferases
  • Alias: beta4Gal-T1
  • Previous Names: GGTB2
  • Alias Names: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1

B4GALT1, beta-1,4-galactosyltransferase 1 Target Infomation by Species

HumanMouseRat

Human B4GALT1 Target Information

  • Target Symbol: B4GALT1, beta-1,4-galactosyltransferase 1
  • Alias:
    • B4GAL-T1
    • beta-1,4-GalTase 1
    • beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
    • beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
    • beta4Gal-T1
    • CDG2D
    • DKFZp686N19253
    • GGTB2
    • glycoprotein-4-beta-galactosyltransferase 2
    • GT1
    • GTB
    • lactose synthase
    • MGC50983
    • N-acetyllactosamine synthase
    • nal synthase
    • neolactotriaosylceramide beta-1,4-galactosyltransferase
    • UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
    • UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
    • UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
  • NCBI_Gene: 2683
  • UniProtKB: P15291

Human B4GALT1 Predicted Functions

Enables several functions, including UDP-galactosyltransferase activity; manganese ion binding activity; and tubulin binding activity. Involved in oligosaccharide biosynthetic process and protein N-linked glycosylation. Located in several cellular components, including Golgi trans cisterna; basolateral plasma membrane; and brush border membrane. Implicated in congenital disorder of glycosylation and congenital disorder of glycosylation type IId.

Mouse B4galt1 Target Information

Mouse B4galt1 Predicted Functions

Enables beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; cytoskeletal protein binding activity; and laminin binding activity involved in cell-matrix adhesion. Involved in cell-matrix adhesion. Acts upstream of or within several processes, including lactose biosynthetic process; single fertilization; and tube morphogenesis. Located in Golgi apparatus; cell surface; and plasma membrane. Is expressed in several structures, including alimentary system; genitourinary system; hemolymphoid system; liver; and nervous system. Used to study IgA glomerulonephritis and endocrine system disease. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation and congenital disorder of glycosylation type IId. Orthologous to human B4GALT1 (beta-1,4-galactosyltransferase 1).

Rat B4galt1 Target Information

  • Target Symbol: B4galt1, beta-1,4-galactosyltransferase 1
  • Alias:
    • b4Gal-T1
    • B4galt1_mapped
    • beta-1,4-GalTase 1
    • beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
    • beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
    • beta4Gal-T1
    • Ggtb2
    • Glycoprotein-4-beta-galactosyltransferase 2
    • N-acetyllactosamine synthase
    • nal synthase
    • neolactotriaosylceramide beta-1,4-galactosyltransferase
    • UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
    • UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
    • UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (mapped)
    • UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
  • NCBI_Gene: 24390

Rat B4galt1 Predicted Functions

Predicted to enable several functions, including UDP-galactosyltransferase activity; laminin binding activity involved in cell-matrix adhesion; and tubulin binding activity. Involved in response to wounding. Predicted to be located in several cellular components, including Golgi trans cisterna; basolateral plasma membrane; and brush border membrane. Predicted to be active in Golgi apparatus and plasma membrane. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation and congenital disorder of glycosylation type IId. Orthologous to human B4GALT1 (beta-1,4-galactosyltransferase 1).

B4GALT1 Target News

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