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Human B4GALT1/GGTB2 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 0.06 ng/mL |
| Range | 1.56 ng/mL – 100 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 105% – 120% |
| Average Recovery | 1.15 |
| Intra Precision | 2.9% – 4.4% |
| Inter-Precision | 3.0% – 3.2% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-B4GALT1/GGTB2 monoclonal antibody Human B4GALT1/GGTB2 freeze-dried standard B4GALT1/GGTB2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human B4GALT1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, B4GALT1 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of B4GALT1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
B4GALT1
B4GALT1 Target Infomation Overview
- Target Symbol: B4GALT1, beta-1,4-galactosyltransferase 1
- Gene Groups: Beta 4-glycosyltransferases
- Alias: beta4Gal-T1
- Previous Names: GGTB2
- Alias Names: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
B4GALT1, beta-1,4-galactosyltransferase 1 Target Infomation by Species
HumanMouseRat
Human B4GALT1 Target Information
- Target Symbol: B4GALT1, beta-1,4-galactosyltransferase 1
- Alias:
- B4GAL-T1
- beta-1,4-GalTase 1
- beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
- beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
- beta4Gal-T1
- CDG2D
- DKFZp686N19253
- GGTB2
- glycoprotein-4-beta-galactosyltransferase 2
- GT1
- GTB
- lactose synthase
- MGC50983
- N-acetyllactosamine synthase
- nal synthase
- neolactotriaosylceramide beta-1,4-galactosyltransferase
- UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
- UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
- NCBI_Gene: 2683
- UniProtKB: P15291
Human B4GALT1 Predicted Functions
Enables several functions, including UDP-galactosyltransferase activity; manganese ion binding activity; and tubulin binding activity. Involved in oligosaccharide biosynthetic process and protein N-linked glycosylation. Located in several cellular components, including Golgi trans cisterna; basolateral plasma membrane; and brush border membrane. Implicated in congenital disorder of glycosylation and congenital disorder of glycosylation type IId.
Mouse B4galt1 Target Information
- Target Symbol: B4galt1, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
- Alias:
- AA407245
- B-1,4-GalT1
- b1,4-Galactosyltransferase I
- beta 1,4-Galactosyltransferase I
- beta-1,4-GalT1
- expressed sequence AA407245
- galactosyltransferase 2 beta 1, 4
- GalT
- Ggtb
- Ggtb2
- glycoprotein galactosyltransferase beta 1, 4
- NCBI_Gene: 14595
Mouse B4galt1 Predicted Functions
Enables beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; cytoskeletal protein binding activity; and laminin binding activity involved in cell-matrix adhesion. Involved in cell-matrix adhesion. Acts upstream of or within several processes, including lactose biosynthetic process; single fertilization; and tube morphogenesis. Located in Golgi apparatus; cell surface; and plasma membrane. Is expressed in several structures, including alimentary system; genitourinary system; hemolymphoid system; liver; and nervous system. Used to study IgA glomerulonephritis and endocrine system disease. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation and congenital disorder of glycosylation type IId. Orthologous to human B4GALT1 (beta-1,4-galactosyltransferase 1).
Rat B4galt1 Target Information
- Target Symbol: B4galt1, beta-1,4-galactosyltransferase 1
- Alias:
- b4Gal-T1
- B4galt1_mapped
- beta-1,4-GalTase 1
- beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
- beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
- beta4Gal-T1
- Ggtb2
- Glycoprotein-4-beta-galactosyltransferase 2
- N-acetyllactosamine synthase
- nal synthase
- neolactotriaosylceramide beta-1,4-galactosyltransferase
- UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (mapped)
- UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
- NCBI_Gene: 24390
Rat B4galt1 Predicted Functions
Predicted to enable several functions, including UDP-galactosyltransferase activity; laminin binding activity involved in cell-matrix adhesion; and tubulin binding activity. Involved in response to wounding. Predicted to be located in several cellular components, including Golgi trans cisterna; basolateral plasma membrane; and brush border membrane. Predicted to be active in Golgi apparatus and plasma membrane. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation and congenital disorder of glycosylation type IId. Orthologous to human B4GALT1 (beta-1,4-galactosyltransferase 1).
