EK1252

Human Bcl-2 ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity50.25 pg/mL
Array Range0.47 ng/mL – 30 ng/mL
Assay Time3.5 h
Recovery90% – 110%
Average Recovery99%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity50.25 pg/mL
Array Range0.47 ng/mL – 30 ng/mL
Assay Time3.5 h
Recovery90% – 110%
Average Recovery99%
Intra Precision4.0% – 4.9%
Inter Precision2.4% – 4.7%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Bcl-2 monoclonal antibody
Human Bcl-2 freeze-dried standard
Bcl-2 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Bcl-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, Bcl-2 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Bcl-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

BCL2

BCL2 Target Infomation Overview

  • Target Symbol: BCL2, BCL2 apoptosis regulator
  • Gene Groups: Protein phosphatase 1 regulatory subunits; BCL2 family
  • Alias: Bcl-2; PPP1R50
  • Alias Names: protein phosphatase 1, regulatory subunit 50; B-cell CLL/lymphoma 2; BCL2, apoptosis regulator

BCL2, BCL2 apoptosis regulator Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human BCL2 Target Information

  • Target Symbol: BCL2, BCL2 apoptosis regulator
  • Alias:
    • apoptosis regulator Bcl-2
    • B-cell CLL/lymphoma 2
    • b-cell leukemia/lymphoma 2
    • Bcl-2
    • Bcl-2a
    • Bcl-2ß
    • BCL2, apoptosis regulator
    • PPP1R50
    • protein phosphatase 1, regulatory subunit 50
  • NCBI_Gene: 596
  • UniProtKB: P10415

Human BCL2 Predicted Functions

Enables several functions, including BH3 domain binding activity; enzyme binding activity; and protein heterodimerization activity. Involved in several processes, including B cell receptor signaling pathway; negative regulation of apoptotic process; and positive regulation of B cell proliferation. Acts upstream of or within several processes, including B cell proliferation; apoptotic signaling pathway; and negative regulation of apoptotic process. Located in endoplasmic reticulum; mitochondrial outer membrane; and nucleus. Part of pore complex. Implicated in several diseases, including carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; prostate cancer; and urinary bladder cancer. Biomarker of several diseases, including cervix uteri carcinoma in situ; ductal carcinoma in situ; gastrointestinal system cancer (multiple); hematologic cancer (multiple); and urinary system cancer (multiple).

Mouse Bcl2 Target Information

Mouse Bcl2 Predicted Functions

Enables protein phosphatase 2A binding activity. Involved in several processes, including hair follicle morphogenesis; mitochondrion organization; and negative regulation of reactive oxygen species metabolic process. Acts upstream of or within several processes, including animal organ development; apoptotic signaling pathway; and negative regulation of programmed cell death. Located in several cellular components, including mitochondrial membrane; myelin sheath; and nuclear membrane. Is expressed in several structures, including central nervous system; genitourinary system; gut; limb; and sensory organ. Used to study oligomeganephronia. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease; carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; and prostate cancer. Orthologous to human BCL2 (BCL2 apoptosis regulator).

Rat Bcl2 Target Information

Rat Bcl2 Predicted Functions

Enables BH domain binding activity. Involved in several processes, including response to peptide; response to purine-containing compound; and response to vitamin. Located in cytosol; mitochondrial crista; and perinuclear region of cytoplasm. Used to study several diseases, including brain ischemia (multiple); end stage renal disease; gastric ulcer; intestinal disease (multiple); and status epilepticus. Biomarker of several diseases, including abdominal obesity-metabolic syndrome 1; brain disease (multiple); hypertension (multiple); intrinsic cardiomyopathy (multiple); and neurodegenerative disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; prostate cancer; and urinary bladder cancer. Orthologous to human BCL2 (BCL2 apoptosis regulator).

Citations for Human Bcl-2 ELISA Kit

There are no citations for this product yet.

If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.

Citations data is updating...

Reviews

There are no reviews yet.

Be the first to review “Human Bcl-2 ELISA Kit”

Your email address will not be published. Required fields are marked *