Human Bcl-2 ELISA Kit
$245.00 – $350.00
Related Target | |
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Species | human |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | 80 μL 1×Assay Buffer and 20 μL sample |
Sensitivity | 50.25 pg/mL |
Array Range | 0.47 ng/mL – 30 ng/mL |
Assay Time | 3.5 h |
Recovery | 90% – 110% |
Average Recovery | 99% |
ELISA Kit Detail Information
Related Target | |
---|---|
Species | human |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | 80 μL 1×Assay Buffer and 20 μL sample |
Sensitivity | 50.25 pg/mL |
Array Range | 0.47 ng/mL – 30 ng/mL |
Assay Time | 3.5 h |
Recovery | 90% – 110% |
Average Recovery | 99% |
Intra Precision | 4.0% – 4.9% |
Inter Precision | 2.4% – 4.7% |
Plate | Detachable 96-well plate |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Bcl-2 monoclonal antibody Human Bcl-2 freeze-dried standard Bcl-2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Bcl-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, Bcl-2 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Bcl-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
BCL2
BCL2 Target Infomation Overview
- Target Symbol: BCL2, BCL2 apoptosis regulator
- Gene Groups: Protein phosphatase 1 regulatory subunits; BCL2 family
- Alias: Bcl-2; PPP1R50
- Alias Names: protein phosphatase 1, regulatory subunit 50; B-cell CLL/lymphoma 2; BCL2, apoptosis regulator
BCL2, BCL2 apoptosis regulator Target Infomation by Species
- Human
- Mouse
- Rat
Human BCL2 Target Information
- Target Symbol: BCL2, BCL2 apoptosis regulator
- Alias:
- apoptosis regulator Bcl-2
- B-cell CLL/lymphoma 2
- b-cell leukemia/lymphoma 2
- Bcl-2
- Bcl-2a
- Bcl-2ß
- BCL2, apoptosis regulator
- PPP1R50
- protein phosphatase 1, regulatory subunit 50
- NCBI_Gene: 596
- UniProtKB: P10415
Human BCL2 Predicted Functions
Enables several functions, including BH3 domain binding activity; enzyme binding activity; and protein heterodimerization activity. Involved in several processes, including B cell receptor signaling pathway; negative regulation of apoptotic process; and positive regulation of B cell proliferation. Acts upstream of or within several processes, including B cell proliferation; apoptotic signaling pathway; and negative regulation of apoptotic process. Located in endoplasmic reticulum; mitochondrial outer membrane; and nucleus. Part of pore complex. Implicated in several diseases, including carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; prostate cancer; and urinary bladder cancer. Biomarker of several diseases, including cervix uteri carcinoma in situ; ductal carcinoma in situ; gastrointestinal system cancer (multiple); hematologic cancer (multiple); and urinary system cancer (multiple).
Mouse Bcl2 Target Information
- Target Symbol: Bcl2, B cell leukemia/lymphoma 2
- Alias:
- AW986256
- Bcl-2
- C430015F12Rik
- D630044D05Rik
- D830018M01Rik
- expressed sequence AW986256
- RIKEN cDNA C430015F12 gene
- RIKEN cDNA D630044D05 gene
- RIKEN cDNA D830018M01 gene
- NCBI_Gene: 12043
Mouse Bcl2 Predicted Functions
Enables protein phosphatase 2A binding activity. Involved in several processes, including hair follicle morphogenesis; mitochondrion organization; and negative regulation of reactive oxygen species metabolic process. Acts upstream of or within several processes, including animal organ development; apoptotic signaling pathway; and negative regulation of programmed cell death. Located in several cellular components, including mitochondrial membrane; myelin sheath; and nuclear membrane. Is expressed in several structures, including central nervous system; genitourinary system; gut; limb; and sensory organ. Used to study oligomeganephronia. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease; carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; and prostate cancer. Orthologous to human BCL2 (BCL2 apoptosis regulator).
Rat Bcl2 Target Information
- Target Symbol: Bcl2, BCL2, apoptosis regulator
- Alias:
- apoptosis regulator Bcl-2
- B cell lymphoma 2 associated oncogene
- B-cell CLL/lymphoma 2
- B-cell leukemia/lymphoma 2
- Bcl-2
- Bcl-2alpha
- Bcl2-like protein
- NCBI_Gene: 24224
Rat Bcl2 Predicted Functions
Enables BH domain binding activity. Involved in several processes, including response to peptide; response to purine-containing compound; and response to vitamin. Located in cytosol; mitochondrial crista; and perinuclear region of cytoplasm. Used to study several diseases, including brain ischemia (multiple); end stage renal disease; gastric ulcer; intestinal disease (multiple); and status epilepticus. Biomarker of several diseases, including abdominal obesity-metabolic syndrome 1; brain disease (multiple); hypertension (multiple); intrinsic cardiomyopathy (multiple); and neurodegenerative disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; prostate cancer; and urinary bladder cancer. Orthologous to human BCL2 (BCL2 apoptosis regulator).
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