Human BID ELISA Kit

$350.00$450.00

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TARGETSBID
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume5 μL
Sensitivity0.51 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery93% – 109%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume5 μL
Sensitivity0.51 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery93% – 109%
Average Recovery1.02
Intra Precision2.3% – 3.0%
Inter-Precision1.7% – 2.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with specific anti-human BID antibody
Human BID freeze-dried standard
Streptavidin-HRP labeled human BID detect Antibody
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Standard Diluent
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human BID antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, BID present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of BID in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

BID

Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Sepsis
Transplantation
Tumor Marker

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