Human CD117/c-kit ELISA Kit

$245.00 – $350.00

Related Target	KIT
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	1.91 pg/mL
Array Range	23.44 – 1500 pg/ml
Assay Time	3.5 h
Recovery	94% - 119%
Average Recovery	108%

ELISA Kit Detail Information

Related Target	KIT
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	1.91 pg/mL
Array Range	23.44 – 1500 pg/ml
Assay Time	3.5 h
Recovery	94% - 119%
Average Recovery	108%
Intra Precision	7.6% – 8.4%
Inter Precision	8.4% – 8.4%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with mouse anti-human CD117 capture antibody Human CD117 freeze-dried standard Human CD117 detect Antibody Streptavidin-HRP Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film Standard Diluent
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CD117 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CD117 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CD117 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

KIT

KIT Target Infomation Overview

Target Symbol: KIT, KIT proto-oncogene, receptor tyrosine kinase
Gene Groups: Receptor tyrosine kinases; CD molecules; Immunoglobulin like domain containing
Alias: CD117; SCFR; C-Kit
Previous Names: PBT
Alias Names: mast/stem cell growth factor receptor Kit; piebald trait; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

KIT, KIT proto-oncogene, receptor tyrosine kinase Target Infomation by Species

Human
Mouse
Rat

Human KIT Target Information

Target Symbol: KIT, KIT proto-oncogene, receptor tyrosine kinase
Alias:
- C-Kit
- c-Kit protooncogene
- CD117
- KIT proto-oncogene receptor tyrosine kinase
- mast/stem cell growth factor receptor
- mast/stem cell growth factor receptor Kit
- MASTC
- p145 c-kit
- PBT
- piebald trait
- piebald trait protein
- proto-oncogene c-Kit
- proto-oncogene tyrosine-protein kinase Kit
- SCFR
- soluble KIT variant 1
- tyrosine-protein kinase Kit
- v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
- v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
NCBI_Gene: 3815
UniProtKB: P10721

Human KIT Predicted Functions

Enables cytokine binding activity; protein homodimerization activity; and transmembrane receptor protein tyrosine kinase activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Located in extracellular space; fibrillar center; and plasma membrane. Implicated in several diseases, including acute myeloid leukemia; gastrointestinal stromal tumor; germ cell cancer (multiple); piebaldism; and renal cell carcinoma. Biomarker of several diseases, including Hirschsprung’s disease; invasive ductal carcinoma; oxyphilic adenoma; reproductive organ cancer (multiple); and urinary system cancer (multiple).

Mouse Kit Target Information

Target Symbol: Kit, KIT proto-oncogene receptor tyrosine kinase
Alias:
- belly-spot
- Bs
- c-KIT
- CD117
- dominant spotting
- Dominant white spotting
- Fdc
- gsf spotted coat 1
- gsf spotted coat 5
- Gsfsco1
- Gsfsco5
- Gsfsow3
- phenotype like Sl or W 3
- SCO1
- SCO5
- SOW3
- spotted sterile male
- Ssm
- Steel Factor Receptor
- Tr-kit
- W
NCBI_Gene: 16590

Mouse Kit Predicted Functions

Enables SH2 domain binding activity; protease binding activity; and stem cell factor receptor activity. Involved in several processes, including detection of mechanical stimulus involved in sensory perception of sound; erythropoietin-mediated signaling pathway; and hemopoiesis. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; hemopoiesis; and positive regulation of macromolecule metabolic process. Located in cell-cell junction and external side of plasma membrane. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; embryo mesenchyme; and genitourinary system. Used to study gastrointestinal stromal tumor; macrocytic anemia; mastocytosis; and piebaldism. Human ortholog(s) of this gene implicated in several diseases, including acute myeloid leukemia; gastrointestinal stromal tumor; germ cell cancer (multiple); piebaldism; and renal cell carcinoma. Orthologous to human KIT (KIT proto-oncogene, receptor tyrosine kinase).

Rat Kit Target Information

Target Symbol: Kit, KIT proto-oncogene receptor tyrosine kinase
Alias:
- c-kit receptor tyrosine kinase
- Kit oncogene
- mast/stem cell growth factor receptor
- mast/stem cell growth factor receptor Kit
- protein kinase
- v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
NCBI_Gene: 64030

Rat Kit Predicted Functions

Enables protein tyrosine kinase activity. Involved in several processes, including gamete generation; positive regulation of gastro-intestinal system smooth muscle contraction; and protein phosphorylation. Located in acrosomal vesicle; cytoplasmic side of plasma membrane; and external side of plasma membrane. Used to study aplastic anemia and male infertility. Biomarker of acute pancreatitis and gastroschisis. Human ortholog(s) of this gene implicated in several diseases, including acute myeloid leukemia; gastrointestinal stromal tumor; germ cell cancer (multiple); piebaldism; and renal cell carcinoma. Orthologous to human KIT (KIT proto-oncogene, receptor tyrosine kinase).

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