Human FGF-21 ELISA Kit

$350.00$450.00

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TARGETSFGF21
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.19 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery81% – 121%
Average Recovery1.04
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.19 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery81% – 121%
Average Recovery1.04
Intra Precision2.7% – 4.8%
Inter-Precision3.1% – 6.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-FGF-21 monoclonal antibody
Human FGF-21 freeze-dried standard
FGF-21 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human FGF-21 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, FGF-21 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of FGF-21 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

FGF21

FGF21 Target Infomation Overview

  • Target Symbol: FGF21, fibroblast growth factor 21
  • Gene Groups: Fibroblast growth factor family

FGF21, fibroblast growth factor 21 Target Infomation by Species

HumanMouseRat

Human FGF21 Target Information

Human FGF21 Predicted Functions

Predicted to enable fibroblast growth factor receptor binding activity and growth factor activity. Involved in positive regulation of ERK1 and ERK2 cascade and positive regulation of glucose import. Predicted to be located in extracellular region. Predicted to be active in cytoplasm and extracellular space. Biomarker of atherosclerosis; chronic kidney disease; and fatty liver disease.

Mouse Fgf21 Target Information

Mouse Fgf21 Predicted Functions

Predicted to enable fibroblast growth factor receptor binding activity and growth factor activity. Involved in positive regulation of cold-induced thermogenesis. Acts upstream of or within positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway and positive regulation of cell population proliferation. Located in extracellular space. Is expressed in several structures, including alimentary system; cerebellum; integumental system; limb; and skeleton. Orthologous to human FGF21 (fibroblast growth factor 21).

Rat Fgf21 Target Information

Rat Fgf21 Predicted Functions

Predicted to enable fibroblast growth factor receptor binding activity and growth factor activity. Involved in several processes, including cellular response to glucagon stimulus; endothelial cell apoptotic process; and negative regulation of cell death. Located in extracellular space. Orthologous to human FGF21 (fibroblast growth factor 21).

FGF21 Target News

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