Human HSP27/HSPB1 ELISA Kit

$350.00$450.00

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TARGETSHSPB1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity5.86 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery92% – 114%
Average Recovery1.03
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity5.86 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery92% – 114%
Average Recovery1.03
Intra Precision3.3% – 6.9%
Inter-Precision3.2%-4.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-HSP27/HSPB1 monoclonal antibody
Human HSP27/HSPB1 freeze-dried standard
Human HSP27/HSPB1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human HSP27 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, HSP27 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of HSP27 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

HSPB1

HSPB1 Target Infomation Overview

  • Target Symbol: HSPB1, heat shock protein family B (small) member 1
  • Gene Groups: Small heat shock proteins
  • Alias: HSP27; HSP28; Hs.76067; Hsp25; CMT2F
  • Alias Names: heat shock 27kD protein 1; heat shock 27kDa protein 1

HSPB1, heat shock protein family B (small) member 1 Target Infomation by Species

HumanMouseRat

Human HSPB1 Target Information

  • Target Symbol: HSPB1, heat shock protein family B (small) member 1
  • Alias:
    • 28 kDa heat shock protein
    • CMT2F
    • DKFZp586P1322
    • epididymis secretory protein Li 102
    • estrogen-regulated 24 kDa protein
    • heat shock 27 kDa protein
    • heat shock 27kD protein 1
    • heat shock 27kDa protein 1
    • heat shock protein beta-1
    • HEL-S-102
    • HMN2B
    • HS.76067
    • HSP 27
    • Hsp25
    • HSP27
    • HSP28
    • putative heat shock protein family B member 1 isoform
    • SRP27
    • stress-responsive protein 27
  • NCBI_Gene: 3315
  • UniProtKB: P04792

Human HSPB1 Predicted Functions

Enables several functions, including protein folding chaperone; protein homodimerization activity; and protein kinase binding activity. Involved in several processes, including anterograde axonal protein transport; positive regulation of angiogenesis; and positive regulation of endothelial cell migration. Located in cytoplasm and nucleus. Implicated in Charcot-Marie-Tooth disease axonal type 2F; distal hereditary motor neuropathy type 2B; pancreatic cancer; and sarcoma. Biomarker of focal segmental glomerulosclerosis and rheumatoid arthritis.

Mouse Hspb1 Target Information

Mouse Hspb1 Predicted Functions

Enables protein kinase C binding activity and protein kinase C inhibitor activity. Involved in negative regulation of protein kinase activity; positive regulation of cytokine production; and regulation of intracellular signal transduction. Acts upstream of or within negative regulation of apoptotic signaling pathway. Located in Z disc; nucleus; and plasma membrane. Is expressed in several structures, including alimentary system; brain; early conceptus; genitourinary system; and heart. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease axonal type 2F; distal hereditary motor neuropathy type 2B; pancreatic cancer; and sarcoma. Orthologous to human HSPB1 (heat shock protein family B (small) member 1).

Rat Hspb1 Target Information

  • Target Symbol: Hspb1, heat shock protein family B (small) member 1
  • Alias:
    • heat shock 27 kDa protein
    • heat shock 27 kDa protein 1
    • heat shock 27kDa protein 1
    • heat shock protein 1
    • heat shock protein B1
    • heat shock protein beta-1
    • HSP 27
    • Hsp25
    • Hsp27
  • NCBI_Gene: 24471

Rat Hspb1 Predicted Functions

Enables ubiquitin binding activity. Involved in several processes, including cellular response to butyrate; cellular response to interleukin-11; and response to ischemia. Located in several cellular components, including myofibril; perikaryon; and postsynaptic density membrane. Part of proteasome complex. Biomarker of Alzheimer’s disease and transient cerebral ischemia. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease axonal type 2F; distal hereditary motor neuropathy type 2B; pancreatic cancer; and sarcoma. Orthologous to human HSPB1 (heat shock protein family B (small) member 1).

HSPB1 Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Tumor Marker

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