Human IL-13 ELISA Kit

$350.00$450.00

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TARGETSIL13
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.90 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery82% – 110%
Average Recovery0.98
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.90 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery82% – 110%
Average Recovery0.98
Intra Precision1.7% – 2.0%
Inter-Precision2.2% – 4.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-13 monoclonal antibody
Human IL-13 freeze-dried standard
IL-13 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-13 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-13 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-13 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL13

IL13 Target Infomation Overview

  • Target Symbol: IL13, interleukin 13
  • Gene Groups: Interleukins
  • Alias: P600; IL-13; ALRH; BHR1; MGC116786; MGC116788; MGC116789
  • Alias Names: allergic rhinitis; Bronchial hyperresponsiveness-1 (bronchial asthma)

IL13, interleukin 13 Target Infomation by Species

HumanMouseRat

Human IL13 Target Information

  • Target Symbol: IL13, interleukin 13
  • Alias:
    • allergic rhinitis
    • ALRH
    • BHR1
    • Bronchial hyperresponsiveness-1 (bronchial asthma)
    • IL-13
    • interleukin-13
    • MGC116786
    • MGC116788
    • MGC116789
    • P600
  • NCBI_Gene: 3596
  • UniProtKB: P35225

Human IL13 Predicted Functions

Predicted to enable cytokine activity and interleukin-13 receptor binding activity. Involved in several processes, including negative regulation of complement-dependent cytotoxicity; negative regulation of endothelial cell apoptotic process; and positive regulation of interleukin-10 production. Located in extracellular region. Implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Biomarker of several diseases, including Plasmodium falciparum malaria; autoimmune disease (multiple); cystic fibrosis; lung disease (multiple); and scleroderma (multiple).

Mouse Il13 Target Information

Mouse Il13 Predicted Functions

Predicted to enable interleukin-13 receptor binding activity. Involved in positive regulation of cold-induced thermogenesis and positive regulation of macrophage activation. Acts upstream of or within cellular response to cytokine stimulus; inflammatory response; and positive regulation of immune effector process. Located in cytoplasm; external side of plasma membrane; and extracellular space. Is expressed in gut; liver; male reproductive gland or organ; and thymus. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Orthologous to human IL13 (interleukin 13).

Rat Il13 Target Information

Rat Il13 Predicted Functions

Enables interleukin-13 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of cation transmembrane transport. Located in extracellular space. Used to study asthma; extrinsic allergic alveolitis; and lipoid nephrosis. Biomarker of several diseases, including alcoholic hepatitis; middle cerebral artery infarction; peptic esophagitis; prostatic hypertrophy; and pulmonary fibrosis. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Orthologous to human IL13 (interleukin 13).

IL13 Target News

    3.5
    CLDN1 silencing suppresses the proliferation and migration of airway smooth muscle cells by modulating MMP14
    AUTOIMMUNITY
    5.6
    The enrichment of Arg1+ILC2s and ILCregs facilitates the progression of endometriosis: A preliminary study
    INTERNATIONAL IMMUNOPHARMACOLOGY
    6.388
    Silibinin attenuated pseudo-allergic reactions and mast cell degranulation via PLCγ and PI3K/Akt signaling pathway
    PHYTOTHERAPY RESEARCH
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus

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