Human IL-13 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	IL13
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	0.90 pg/mL
Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	82% – 110%
Average Recovery	0.98

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	0.90 pg/mL
Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	82% – 110%
Average Recovery	0.98
Intra Precision	1.7% – 2.0%
Inter-Precision	2.2% – 4.1%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-13 monoclonal antibody Human IL-13 freeze-dried standard IL-13 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-13 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-13 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-13 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL13

IL13 Target Infomation Overview

Target Symbol: IL13, interleukin 13
Gene Groups: Interleukins
Alias: P600; IL-13; ALRH; BHR1; MGC116786; MGC116788; MGC116789
Alias Names: allergic rhinitis; Bronchial hyperresponsiveness-1 (bronchial asthma)

IL13, interleukin 13 Target Infomation by Species

HumanMouseRat

Human IL13 Target Information

Target Symbol: IL13, interleukin 13
Alias:
- allergic rhinitis
- ALRH
- BHR1
- Bronchial hyperresponsiveness-1 (bronchial asthma)
- IL-13
- interleukin-13
- MGC116786
- MGC116788
- MGC116789
- P600
NCBI_Gene: 3596
UniProtKB: P35225

Human IL13 Predicted Functions

Predicted to enable cytokine activity and interleukin-13 receptor binding activity. Involved in several processes, including negative regulation of complement-dependent cytotoxicity; negative regulation of endothelial cell apoptotic process; and positive regulation of interleukin-10 production. Located in extracellular region. Implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Biomarker of several diseases, including Plasmodium falciparum malaria; autoimmune disease (multiple); cystic fibrosis; lung disease (multiple); and scleroderma (multiple).

Mouse Il13 Target Information

Target Symbol: Il13, interleukin 13
Alias:
- Il-13
NCBI_Gene: 16163
- UniprotKB: P20109

Mouse Il13 Predicted Functions

Predicted to enable interleukin-13 receptor binding activity. Involved in positive regulation of cold-induced thermogenesis and positive regulation of macrophage activation. Acts upstream of or within cellular response to cytokine stimulus; inflammatory response; and positive regulation of immune effector process. Located in cytoplasm; external side of plasma membrane; and extracellular space. Is expressed in gut; liver; male reproductive gland or organ; and thymus. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Orthologous to human IL13 (interleukin 13).

Rat Il13 Target Information

Target Symbol: Il13, interleukin 13
Alias:
- IL-13
- interleukin-13
- T-cell activation protein P600
NCBI_Gene: 116553
UniProtKB: P42203

Rat Il13 Predicted Functions

Enables interleukin-13 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of cation transmembrane transport. Located in extracellular space. Used to study asthma; extrinsic allergic alveolitis; and lipoid nephrosis. Biomarker of several diseases, including alcoholic hepatitis; middle cerebral artery infarction; peptic esophagitis; prostatic hypertrophy; and pulmonary fibrosis. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Orthologous to human IL13 (interleukin 13).