Human IL-5 ELISA Kit

$350.00$450.00

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TARGETSIL5
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.76 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery91% – 107%
Average Recovery0.99
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.76 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery91% – 107%
Average Recovery0.99
Intra Precision2.5% – 4.0%
Inter-Precision3.2% – 6.1%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-5 monoclonal antibody
Human IL-5 freeze-dried standard
IL-5 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-5 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-5 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-5 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL5

IL5 Target Infomation Overview

  • Target Symbol: IL5, interleukin 5
  • Gene Groups: Interleukins
  • Alias: IL-5; EDF; TRF
  • Alias Names: interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)

IL5, interleukin 5 Target Infomation by Species

HumanMouseRat

Human IL5 Target Information

  • Target Symbol: IL5, interleukin 5
  • Alias:
    • B cell differentiation factor I
    • B-cell differentiation factor I
    • colony-stimulating factor, eosinophil
    • EDF
    • eosinophil differentiation factor
    • IL-5
    • interleukin-5
    • T-cell replacing factor
    • TRF
  • NCBI_Gene: 3567
  • UniProtKB: P05113

Human IL5 Predicted Functions

Predicted to enable cytokine activity; growth factor activity; and interleukin-5 receptor binding activity. Involved in positive regulation of podosome assembly. Located in extracellular region. Implicated in asthma. Biomarker of several diseases, including Churg-Strauss syndrome; Henoch-Schoenlein purpura; arthritis (multiple); asthma (multiple); and lymphoid leukemia (multiple).

Mouse Il5 Target Information

Mouse Il5 Predicted Functions

Enables cytokine activity. Involved in positive regulation of DNA-binding transcription factor activity; positive regulation of immunoglobulin production; and positive regulation of transcription, DNA-templated. Acts upstream of or within positive regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in colon; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).

Rat Il5 Target Information

  • Target Symbol: Il5, interleukin 5
  • Alias:
    • B-cell growth factor II
    • BCGF-II
    • cytotoxic T-lymphocyte inducer
    • eosinophil differentiation factor
    • IL-5
    • Interleukin 5 (colony-stimulating factor eosinophil)
    • Interleukin 5 (colony-stimulating factor, eosinophil)
    • interleukin-5
    • T-cell replacing factor
    • TRF
  • NCBI_Gene: 24497

Rat Il5 Predicted Functions

Enables cytokine activity and interleukin-5 receptor binding activity. Involved in several processes, including positive regulation of B cell proliferation; positive regulation of eosinophil differentiation; and positive regulation of receptor signaling pathway via JAK-STAT. Located in extracellular space. Used to study asthma. Biomarker of several diseases, including allergic rhinitis; esophageal cancer; middle cerebral artery infarction; peptic esophagitis; and prostatic hypertrophy. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).

IL5 Target News

    3.5
    CLDN1 silencing suppresses the proliferation and migration of airway smooth muscle cells by modulating MMP14
    AUTOIMMUNITY
    4.657
    Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro
    INFLAMMATION
    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Systemic Lupus Erythematosus
    Transplantation

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