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Human IL-5 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 0.76 pg/mL |
| Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 91% – 107% |
| Average Recovery | 0.99 |
| Intra Precision | 2.5% – 4.0% |
| Inter-Precision | 3.2% – 6.1% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-5 monoclonal antibody Human IL-5 freeze-dried standard IL-5 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-5 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-5 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-5 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IL5
IL5 Target Infomation Overview
- Target Symbol: IL5, interleukin 5
- Gene Groups: Interleukins
- Alias: IL-5; EDF; TRF
- Alias Names: interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)
IL5, interleukin 5 Target Infomation by Species
HumanMouseRat
Human IL5 Target Information
- Target Symbol: IL5, interleukin 5
- Alias:
- B cell differentiation factor I
- B-cell differentiation factor I
- colony-stimulating factor, eosinophil
- EDF
- eosinophil differentiation factor
- IL-5
- interleukin-5
- T-cell replacing factor
- TRF
- NCBI_Gene: 3567
- UniProtKB: P05113
Human IL5 Predicted Functions
Predicted to enable cytokine activity; growth factor activity; and interleukin-5 receptor binding activity. Involved in positive regulation of podosome assembly. Located in extracellular region. Implicated in asthma. Biomarker of several diseases, including Churg-Strauss syndrome; Henoch-Schoenlein purpura; arthritis (multiple); asthma (multiple); and lymphoid leukemia (multiple).
Mouse Il5 Target Information
- Target Symbol: Il5, interleukin 5
- Alias:
- Il-5
- NCBI_Gene: 16191
Mouse Il5 Predicted Functions
Enables cytokine activity. Involved in positive regulation of DNA-binding transcription factor activity; positive regulation of immunoglobulin production; and positive regulation of transcription, DNA-templated. Acts upstream of or within positive regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in colon; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).
Rat Il5 Target Information
- Target Symbol: Il5, interleukin 5
- Alias:
- B-cell growth factor II
- BCGF-II
- cytotoxic T-lymphocyte inducer
- eosinophil differentiation factor
- IL-5
- Interleukin 5 (colony-stimulating factor eosinophil)
- Interleukin 5 (colony-stimulating factor, eosinophil)
- interleukin-5
- T-cell replacing factor
- TRF
- NCBI_Gene: 24497
Rat Il5 Predicted Functions
Enables cytokine activity and interleukin-5 receptor binding activity. Involved in several processes, including positive regulation of B cell proliferation; positive regulation of eosinophil differentiation; and positive regulation of receptor signaling pathway via JAK-STAT. Located in extracellular space. Used to study asthma. Biomarker of several diseases, including allergic rhinitis; esophageal cancer; middle cerebral artery infarction; peptic esophagitis; and prostatic hypertrophy. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).
