Human IL-8 ELISA Kit

$350.00$450.00

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TARGETSCXCL8
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.78 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery87% – 111%
Average Recovery0.99
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity0.78 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery87% – 111%
Average Recovery0.99
Intra Precision4.0% – 4.9%
Inter-Precision4.5% – 6.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-8 monoclonal antibody
Human IL-8 freeze-dried standard
IL-8 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-8 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-8 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-8 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CXCL8

Telotristat Etiprate alleviates rheumatoid arthritis by targeting LGALS3 and affecting MAPK signaling
3.752
Profiling of metabolites, proteins, and protein phosphorylation in silica-exposed BEAS-2B epithelial cells
PLoS One
4.9
SMAD2 inhibits pyroptosis of fibroblast-like synoviocytes and secretion of inflammatory factors via the TGF-β pathway in rheumatoid arthritis
ARTHRITIS RESEARCH & THERAPY
28.2
An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma
Cancer Discovery
12.469
Chordoma recruits and polarizes tumor-associated macrophages via secreting CCL5 to promote malignant progression
Journal for ImmunoTherapy of Cancer
9.2
Serine deficiency exacerbates psoriatic skin inflammation by regulating S-adenosyl methionine-dependent DNA methylation and NF-κB signaling activation in keratinocytes
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
8.185
CSF2 upregulates CXCL3 expression in adipocytes to promote metastasis of breast cancer via the FAK signaling pathway
Journal of Molecular Cell Biology
9
Airway epithelial cGAS inhibits LPS-induced acute lung injury through CREB signaling
Cell Death & Disease
4.803
The Natural Alkaloid (−)-N-Hydroxyapiosporamide Suppresses Colorectal Tumor Progression as an NF-κB Pathway Inhibitor by Targeting the TAK1–TRAF6 Complex
JOURNAL OF NATURAL PRODUCTS
3.5
Potential salivary and serum biomarkers for burning mouth syndrome and their relationship with anxiety/depression
Journal of Dental Sciences
5.486
Epstein-Barr virus envelope glycoprotein 110 inhibits NF-κB activation by interacting with NF-κB subunit p65
JOURNAL OF BIOLOGICAL CHEMISTRY
5.714
Acteoside alleviates asthma by modulating ROS-responsive NF-κB/MAPK signaling pathway
INTERNATIONAL IMMUNOPHARMACOLOGY
5.714
4-Iodo-6-phenylpyrimidine (4-IPP) suppresses fibroblast-like synoviocyte- mediated inflammation and joint destruction associated with rheumatoid arthritis
INTERNATIONAL IMMUNOPHARMACOLOGY
6.449
Polygonatum kingianum saponins delay cellular senescence through SASP down-regulation and prolong the healthy lifespan of Caenorhabditis elegans by activating sir-2.1/autophagy
INDUSTRIAL CROPS AND PRODUCTS
8.025
Effect of fermentation modification on the physicochemical characteristics and anti-aging related activities of Polygonatum kingianum polysaccharides
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
10.723
Multifunctional chondroitin sulfate based hydrogels for promoting infected diabetic wounds healing by chemo-photothermal antibacterial and cytokine modulation
CARBOHYDRATE POLYMERS
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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