Human Layilin/LAYN ELISA Kit

$350.00$450.00

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TARGETSLAYN
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity4.25 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery80% – 111%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity4.25 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery80% – 111%
Average Recovery0.97
Intra Precision2.7% – 3.8%
Inter-Precision2.6% – 4.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Layilin/LAYN monoclonal antibody
Human Layilin/LAYN freeze-dried standard
Layilin/LAYN detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human LAYN antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, LAYN present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of LAYN in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

LAYN

LAYN Target Infomation Overview

  • Target Symbol: LAYN, layilin
  • Gene Groups: C-type lectin domain containing
  • Alias: FLJ30977; FLJ31092

LAYN, layilin Target Infomation by Species

HumanMouseRat

Human LAYN Target Information

Human LAYN Predicted Functions

Enables hyaluronic acid binding activity. Located in focal adhesion.

Mouse Layn Target Information

Mouse Layn Predicted Functions

Predicted to enable hyaluronic acid binding activity. Predicted to be located in membrane. Predicted to be integral component of membrane. Is expressed in brain and trigeminal ganglion. Orthologous to human LAYN (layilin).

Rat Layn Target Information

Rat Layn Predicted Functions

Predicted to enable hyaluronic acid binding activity. Predicted to be integral component of membrane. Orthologous to human LAYN (layilin).

LAYN Target News

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