Human Layilin/LAYN ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	LAYN
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	4.25 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	80% – 111%
Average Recovery	0.97

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	4.25 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	80% – 111%
Average Recovery	0.97
Intra Precision	2.7% – 3.8%
Inter-Precision	2.6% – 4.4%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Layilin/LAYN monoclonal antibody Human Layilin/LAYN freeze-dried standard Layilin/LAYN detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human LAYN antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, LAYN present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of LAYN in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

LAYN

LAYN Target Infomation Overview

Target Symbol: LAYN, layilin
Gene Groups: C-type lectin domain containing
Alias: FLJ30977; FLJ31092

LAYN, layilin Target Infomation by Species

HumanMouseRat

Human LAYN Target Information

Target Symbol: LAYN, layilin
Alias:
- FLJ30977
- FLJ31092
NCBI_Gene: 143903
UniProtKB: Q6UX15

Human LAYN Predicted Functions

Enables hyaluronic acid binding activity. Located in focal adhesion.

Mouse Layn Target Information

Target Symbol: Layn, layilin
Alias:
- E030012M19Rik
- gene model 511, (NCBI)
- Gm511
- LOC244864
- RIKEN cDNA E030012M19 gene
NCBI_Gene: 244864

Mouse Layn Predicted Functions

Predicted to enable hyaluronic acid binding activity. Predicted to be located in membrane. Predicted to be integral component of membrane. Is expressed in brain and trigeminal ganglion. Orthologous to human LAYN (layilin).