Human MMP-7 ELISA Kit

$350.00$450.00

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TARGETSMMP7
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity4.90 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery82% – 104%
Average Recovery0.92
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity4.90 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery82% – 104%
Average Recovery0.92
Intra Precision3.2% – 4.2%
Inter-Precision3.5% – 4.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-7 monoclonal antibody
Human MMP-7 freeze-dried standard
MMP-7 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MMP-7 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-7 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-7 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MMP7

MMP7 Target Infomation Overview

  • Target Symbol: MMP7, matrix metallopeptidase 7
  • Gene Groups: M10 matrix metallopeptidases
  • Alias: PUMP-1
  • Previous Names: MPSL1
  • Alias Names: matrilysin; matrix metalloproteinase 7 (matrilysin, uterine)

MMP7, matrix metallopeptidase 7 Target Infomation by Species

HumanMouseRat

Human MMP7 Target Information

  • Target Symbol: MMP7, matrix metallopeptidase 7
  • Alias:
    • matrilysin
    • matrin
    • matrix metallopeptidase 7 (matrilysin, uterine)
    • matrix metalloproteinase 7
    • matrix metalloproteinase 7 (matrilysin, uterine)
    • matrix metalloproteinase-7
    • MMP-7
    • MPSL1
    • PUMP-1
    • pump-1 protease
    • uterine matrilysin
    • uterine metalloproteinase
  • NCBI_Gene: 4316
  • UniProtKB: P09237

Human MMP7 Predicted Functions

Enables endopeptidase activity and metallopeptidase activity. Involved in positive regulation of cell migration and proteolysis. Acts upstream of or within membrane protein ectodomain proteolysis and membrane protein intracellular domain proteolysis. Located in extracellular exosome. Biomarker of endometrial cancer; endometrial hyperplasia; glioblastoma; and lung non-small cell carcinoma.

Mouse Mmp7 Target Information

Mouse Mmp7 Predicted Functions

Enables peptidase activity. Acts upstream of or within several processes, including defense response to bacterium; proteolysis; and response to xenobiotic stimulus. Predicted to be located in cell surface. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; hemolymphoid system gland; integumental system; liver and biliary system; and reproductive system. Orthologous to human MMP7 (matrix metallopeptidase 7).

Rat Mmp7 Target Information

  • Target Symbol: Mmp7, matrix metallopeptidase 7
  • Alias:
    • matrilysin
    • matrin
    • matrix metalloproteinase 7
    • Matrix metalloproteinase 7 (matrilysin)
    • matrix metalloproteinase-7
    • MMP-7
    • MPMM
    • pump-1 protease
    • uterine metalloproteinase
  • NCBI_Gene: 25335
  • UniProtKB: P50280

Rat Mmp7 Predicted Functions

Enables heparin binding activity. Involved in several processes, including cellular response to mechanical stimulus; estrous cycle; and maternal process involved in female pregnancy. Located in cell surface and extracellular space. Used to study hypertension and prostate carcinoma. Biomarker of bladder neck obstruction; coronary restenosis; hypertension; prostate cancer; and renal hypertension. Orthologous to human MMP7 (matrix metallopeptidase 7).

MMP7 Target News

    5.86
    Urinary matrix metalloproteinase-7 is a sensitive biomarker to evaluate renal tubular injury in patients with minimal change disease and focal segmental glomerulosclerosis
    Clinical Kidney Journal

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