Human MMP-8 ELISA Kit

$350.00$450.00

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TARGETSMMP8
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity12.66 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery88% – 107%
Average Recovery0.97
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity12.66 pg/mL
Range0.16 ng/mL – 10 ng/mL
Assay Time3.5 h
Recovery88% – 107%
Average Recovery0.97
Intra Precision3.1% – 4.5%
Inter-Precision4.6% – 7.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MMP-8 monoclonal antibody
Human MMP-8 freeze-dried standard
MMP-8 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human MMP-8 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MMP-8 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of MMP-8 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

MMP8

MMP8 Target Infomation Overview

  • Target Symbol: MMP8, matrix metallopeptidase 8
  • Gene Groups: M10 matrix metallopeptidases
  • Previous Names: CLG1
  • Alias Names: matrix metalloproteinase 8 (neutrophil collagenase)

MMP8, matrix metallopeptidase 8 Target Infomation by Species

HumanMouseRat

Human MMP8 Target Information

  • Target Symbol: MMP8, matrix metallopeptidase 8
  • Alias:
    • CLG1
    • collagenase 2
    • HNC
    • matrix metalloproteinase 8 (neutrophil collagenase)
    • matrix metalloproteinase-8
    • MMP-8
    • neutrophil collagenase
    • PMN leukocyte collagenase
    • PMNL collagenase
    • PMNL-CL
  • NCBI_Gene: 4317
  • UniProtKB: P22894

Human MMP8 Predicted Functions

Enables serine-type endopeptidase activity. Involved in endodermal cell differentiation and proteolysis. Located in extracellular space. Implicated in breast cancer and carotid artery disease. Biomarker of COVID-19; aortic aneurysm (multiple); arthritis (multiple); breast cancer; and female reproductive organ cancer (multiple).

Mouse Mmp8 Target Information

Mouse Mmp8 Predicted Functions

Predicted to enable endopeptidase activity and metal ion binding activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of intracellular signal transduction; and regulation of cytokine production. Located in extracellular space. Is expressed in several structures, including central nervous system; genitourinary system; hindlimb digit mesenchyme; musculature; and skeleton. Human ortholog(s) of this gene implicated in breast cancer and carotid artery disease. Orthologous to human MMP8 (matrix metallopeptidase 8).

Rat Mmp8 Target Information

Rat Mmp8 Predicted Functions

Enables calcium ion binding activity; metallopeptidase activity; and zinc ion binding activity. Involved in several processes, including positive regulation of neuroinflammatory response; positive regulation of nitric oxide biosynthetic process; and positive regulation of tumor necrosis factor production. Located in extracellular space. Biomarker of congestive heart failure. Human ortholog(s) of this gene implicated in breast cancer and carotid artery disease. Orthologous to human MMP8 (matrix metallopeptidase 8).

MMP8 Target News

    Osteoarthrosis & Rheumatoid Arthritis

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