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Human Osteoprotegerin/OPG ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 1.36 pg/mL |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 94% – 114% |
| Average Recovery | 1.03 |
| Intra Precision | 4.2% – 5.7% |
| Inter-Precision | 6.4% – 7.5% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Osteoprotegerin/OPGA monoclonal antibody Human Osteoprotegerin/OPGA freeze-dried standard Osteoprotegerin/OPGA detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human OPG antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, OPG present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of OPG in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
TNFRSF11B
TNFRSF11B Target Infomation Overview
- Target Symbol: TNFRSF11B, TNF receptor superfamily member 11b
- Gene Groups: Tumor necrosis factor receptor superfamily
- Alias: OCIF; TR1
- Previous Names: OPG
- Alias Names: osteoclastogenesis inhibitory factor; osteoprotegerin; tumor necrosis factor receptor superfamily, member 11b
TNFRSF11B, TNF receptor superfamily member 11b Target Infomation by Species
HumanMouseRat
Human TNFRSF11B Target Information
- Target Symbol: TNFRSF11B, TNF receptor superfamily member 11b
- Alias:
- MGC29565
- OCIF
- OPG
- osteoclastogenesis inhibitory factor
- osteoprotegerin
- Paget disease of bone 5
- PDB5
- TR1
- tumor necrosis factor receptor superfamily member 11B
- tumor necrosis factor receptor superfamily, member 11b
- NCBI_Gene: 4982
- UniProtKB: O00300
Human TNFRSF11B Predicted Functions
Predicted to enable cytokine activity and signaling receptor activity. Predicted to be involved in skeletal system development. Predicted to act upstream of or within extracellular matrix organization and negative regulation of odontogenesis of dentin-containing tooth. Located in extracellular space. Implicated in Paget’s disease of bone and osteoarthritis. Biomarker of several diseases, including artery disease (multiple); end stage renal disease; hereditary arterial and articular multiple calcification syndrome; lupus nephritis; and type 2 diabetes mellitus.
Mouse Tnfrsf11b Target Information
- Target Symbol: Tnfrsf11b, tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
- Alias:
- OCIF
- OPG
- Opg
- osteoclastogenesis inhibitory factor
- osteoprotegerin
- TR1
- NCBI_Gene: 18383
Mouse Tnfrsf11b Predicted Functions
Acts upstream of or within extracellular matrix organization and negative regulation of odontogenesis of dentin-containing tooth. Located in extracellular matrix. Is expressed in several structures, including arterial system; bone; gut; lung; and placenta. Used to study osteoporosis. Human ortholog(s) of this gene implicated in Paget’s disease of bone and osteoarthritis. Orthologous to human TNFRSF11B (TNF receptor superfamily member 11b).
Rat Tnfrsf11b Target Information
- Target Symbol: Tnfrsf11b, TNF receptor superfamily member 11B
- Alias:
- MGC93568
- Opg
- Osteoprotegerin
- tumor necrosis factor receptor superfamily member 11B
- tumor necrosis factor receptor superfamily, member 11b
- tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
- NCBI_Gene: 25341
- UniProtKB: O08727
Rat Tnfrsf11b Predicted Functions
Predicted to enable cytokine activity. Involved in several processes, including negative regulation of bone resorption; negative regulation of odontogenesis of dentin-containing tooth; and response to estrogen. Located in extracellular space. Biomarker of congestive heart failure; myocarditis; and periapical periodontitis. Human ortholog(s) of this gene implicated in Paget’s disease of bone and osteoarthritis. Orthologous to human TNFRSF11B (TNF receptor superfamily member 11b).
