Human Osteoprotegerin/OPG ELISA Kit

$350.00$450.00

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TARGETSTNFRSF11B
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity1.36 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery94% – 114%
Average Recovery1.03
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity1.36 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery94% – 114%
Average Recovery1.03
Intra Precision4.2% – 5.7%
Inter-Precision6.4% – 7.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Osteoprotegerin/OPGA monoclonal antibody
Human Osteoprotegerin/OPGA freeze-dried standard
Osteoprotegerin/OPGA detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human OPG antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, OPG present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of OPG in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TNFRSF11B

TNFRSF11B Target Infomation Overview

  • Target Symbol: TNFRSF11B, TNF receptor superfamily member 11b
  • Gene Groups: Tumor necrosis factor receptor superfamily
  • Alias: OCIF; TR1
  • Previous Names: OPG
  • Alias Names: osteoclastogenesis inhibitory factor; osteoprotegerin; tumor necrosis factor receptor superfamily, member 11b

TNFRSF11B, TNF receptor superfamily member 11b Target Infomation by Species

HumanMouseRat

Human TNFRSF11B Target Information

  • Target Symbol: TNFRSF11B, TNF receptor superfamily member 11b
  • Alias:
    • MGC29565
    • OCIF
    • OPG
    • osteoclastogenesis inhibitory factor
    • osteoprotegerin
    • Paget disease of bone 5
    • PDB5
    • TR1
    • tumor necrosis factor receptor superfamily member 11B
    • tumor necrosis factor receptor superfamily, member 11b
  • NCBI_Gene: 4982
  • UniProtKB: O00300

Human TNFRSF11B Predicted Functions

Predicted to enable cytokine activity and signaling receptor activity. Predicted to be involved in skeletal system development. Predicted to act upstream of or within extracellular matrix organization and negative regulation of odontogenesis of dentin-containing tooth. Located in extracellular space. Implicated in Paget’s disease of bone and osteoarthritis. Biomarker of several diseases, including artery disease (multiple); end stage renal disease; hereditary arterial and articular multiple calcification syndrome; lupus nephritis; and type 2 diabetes mellitus.

Mouse Tnfrsf11b Target Information

Mouse Tnfrsf11b Predicted Functions

Acts upstream of or within extracellular matrix organization and negative regulation of odontogenesis of dentin-containing tooth. Located in extracellular matrix. Is expressed in several structures, including arterial system; bone; gut; lung; and placenta. Used to study osteoporosis. Human ortholog(s) of this gene implicated in Paget’s disease of bone and osteoarthritis. Orthologous to human TNFRSF11B (TNF receptor superfamily member 11b).

Rat Tnfrsf11b Target Information

  • Target Symbol: Tnfrsf11b, TNF receptor superfamily member 11B
  • Alias:
    • MGC93568
    • Opg
    • Osteoprotegerin
    • tumor necrosis factor receptor superfamily member 11B
    • tumor necrosis factor receptor superfamily, member 11b
    • tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
  • NCBI_Gene: 25341
  • UniProtKB: O08727

Rat Tnfrsf11b Predicted Functions

Predicted to enable cytokine activity. Involved in several processes, including negative regulation of bone resorption; negative regulation of odontogenesis of dentin-containing tooth; and response to estrogen. Located in extracellular space. Biomarker of congestive heart failure; myocarditis; and periapical periodontitis. Human ortholog(s) of this gene implicated in Paget’s disease of bone and osteoarthritis. Orthologous to human TNFRSF11B (TNF receptor superfamily member 11b).

TNFRSF11B Target News

    6.314
    Association of serum anti-carbamylated protein antibodies with disease activity and bone loss in rheumatoid arthritis
    CLINICA CHIMICA ACTA
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis

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