Human PIGF ELISA Kit

$350.00$450.00

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TARGETSPIGF
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.67 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery95% – 122%
Average Recovery1.11
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.67 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery95% – 122%
Average Recovery1.11
Intra Precision2.9% – 3.8%
Inter-Precision1.9% – 5.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PIGF monoclonal antibody
Human PIGF freeze-dried standard
PIGF detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PIGF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, PIGF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of PIGF in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

PIGF

PIGF Target Infomation Overview

  • Target Symbol: PIGF, phosphatidylinositol glycan anchor biosynthesis class F
  • Gene Groups: Phosphatidylinositol glycan anchor biosynthesis
  • Alias Names: phosphatidylinositol glycan, class F

PIGF, phosphatidylinositol glycan anchor biosynthesis class F Target Infomation by Species

HumanMouseRat

Human PIGF Target Information

  • Target Symbol: PIGF, phosphatidylinositol glycan anchor biosynthesis class F
  • Alias:
    • GPI11 homolog
    • MGC32646
    • MGC33136
    • OORS
    • phosphatidylinositol glycan anchor biosynthesis, class F
    • phosphatidylinositol glycan, class f
    • phosphatidylinositol-glycan biosynthesis class F protein
    • PIG-F
  • NCBI_Gene: 5281
  • UniProtKB: Q07326

Human PIGF Predicted Functions

Predicted to enable mannose-ethanolamine phosphotransferase activity. Predicted to be involved in GPI anchor biosynthetic process. Predicted to be integral component of membrane. Predicted to be active in endoplasmic reticulum membrane.

Mouse Pigf Target Information

Mouse Pigf Predicted Functions

Predicted to enable mannose-ethanolamine phosphotransferase activity. Involved in GPI anchor biosynthetic process. Located in endoplasmic reticulum membrane. Is expressed in several structures, including alimentary system; brain; respiratory system; sensory organ; and urinary system. Orthologous to human PIGF (phosphatidylinositol glycan anchor biosynthesis class F).

Rat Pigf Target Information

  • Target Symbol: Pigf, phosphatidylinositol glycan anchor biosynthesis, class F
  • Alias:
    • LOC681086
    • phosphatidylinositol-glycan biosynthesis class F protein
    • similar to Phosphatidylinositol-glycan biosynthesis, class F protein (PIG-F)
  • NCBI_Gene: 681086
  • UniProtKB: D3ZZU7

Rat Pigf Predicted Functions

Predicted to enable mannose-ethanolamine phosphotransferase activity. Predicted to be involved in GPI anchor biosynthetic process. Predicted to be integral component of membrane. Predicted to be active in endoplasmic reticulum membrane. Orthologous to human PIGF (phosphatidylinositol glycan anchor biosynthesis class F).

PIGF Target News

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