Human PRLR ELISA Kit

$350.00$450.00

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TARGETSPRLR
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity1.41 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery82% – 112%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity1.41 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery82% – 112%
Average Recovery1.02
Intra Precision2.0% – 2.8%
Inter-Precision1.6% – 3.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PRLR monoclonal antibody
Human PRLR freeze-dried standard
PRLR detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PRLR antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, PRLR present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PRLR in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

PRLR

PRLR Target Infomation Overview

  • Target Symbol: PRLR, prolactin receptor

PRLR, prolactin receptor Target Infomation by Species

HumanMouseRat

Human PRLR Target Information

  • Target Symbol: PRLR, prolactin receptor
  • Alias:
    • delta 4-delta 7/11 truncated prolactin receptor
    • delta 4-SF1b truncated prolactin receptor
    • HPRL
    • hPRL receptor
    • hPRLrI
    • MFAB
    • PRL-R
    • prolactin receptor delta 7/11
    • RI-PRLR
    • secreted prolactin binding protein
  • NCBI_Gene: 5618
  • UniProtKB: P16471

Human PRLR Predicted Functions

Enables peptide hormone binding activity and prolactin receptor activity. Involved in activation of Janus kinase activity; activation of transmembrane receptor protein tyrosine kinase activity; and negative regulation of apoptotic process. Located in cell surface. Implicated in hyperprolactinemia.

Mouse Prlr Target Information

Mouse Prlr Predicted Functions

Enables prolactin receptor activity. Involved in positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including gland development; receptor signaling pathway via JAK-STAT; and response to bacterium. Predicted to be located in cell surface. Predicted to be part of receptor complex. Predicted to be active in external side of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; hemolymphoid system gland; and limb segment. Used to study familial medullary thyroid carcinoma. Human ortholog(s) of this gene implicated in hyperprolactinemia. Orthologous to human PRLR (prolactin receptor).

Rat Prlr Target Information

Rat Prlr Predicted Functions

Enables prolactin receptor activity and protein kinase binding activity. Predicted to be involved in several processes, including activation of protein kinase activity; cytokine-mediated signaling pathway; and positive regulation of cold-induced thermogenesis. Predicted to act upstream of or within several processes, including gland development; receptor signaling pathway via JAK-STAT; and response to bacterium. Predicted to be located in cell surface. Predicted to be part of receptor complex. Predicted to be active in external side of plasma membrane. Human ortholog(s) of this gene implicated in hyperprolactinemia. Orthologous to human PRLR (prolactin receptor).

PRLR Target News

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