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Human RBP4 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 1.05 pg/mL |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 88% – 110% |
| Average Recovery | 1.02 |
| Intra Precision | 3.6% – 5.9% |
| Inter-Precision | 3.2% – 4.4% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-RBP4 monoclonal antibody Human RBP4 freeze-dried standard RBP4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human RBP4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, RBP4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of RBP4 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
RBP4
RBP4 Target Infomation Overview
- Target Symbol: RBP4, retinol binding protein 4
- Gene Groups: Lipocalins
- Alias Names: retinol-binding protein 4, plasma
RBP4, retinol binding protein 4 Target Infomation by Species
HumanMouseRat
Human RBP4 Target Information
- Target Symbol: RBP4, retinol binding protein 4
- Alias:
- MCOPCB10
- plasma retinol-binding protein
- PRBP
- RBP
- RDCCAS
- retinol binding protein 4, plasma
- retinol-binding protein 4
- retinol-binding protein 4, interstitial
- NCBI_Gene: 5950
- UniProtKB: P02753
Human RBP4 Predicted Functions
Enables retinol binding activity. Involved in several processes, including gluconeogenesis; positive regulation of insulin secretion; and retinol metabolic process. Located in extracellular space. Implicated in type 2 diabetes mellitus. Biomarker of type 2 diabetes mellitus.
Mouse Rbp4 Target Information
- Target Symbol: Rbp4, retinol binding protein 4, plasma
- Alias:
- Rbp-4
- retinol binding protein 4, cellular
- NCBI_Gene: 19662
Mouse Rbp4 Predicted Functions
Enables retinol binding activity. Involved in several processes, including animal organ development; gluconeogenesis; and negative regulation of cardiac muscle cell proliferation. Acts upstream of or within several processes, including detection of light stimulus involved in visual perception; retinoid metabolic process; and terpenoid transport. Located in extracellular space. Is expressed in several structures, including adipose tissue; brain; genitourinary system; gut; and hindlimb. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human RBP4 (retinol binding protein 4).
Rat Rbp4 Target Information
- Target Symbol: Rbp4, retinol binding protein 4
- Alias:
- plasma retinol-binding protein
- PRBP
- RBP
- RBPA
- retinol binding protein 4, plasma
- retinol-binding protein 4
- NCBI_Gene: 25703
Rat Rbp4 Predicted Functions
Enables retinol binding activity. Involved in response to ethanol and retinol transport. Located in extracellular space. Part of protein-containing complex. Biomarker of obesity and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human RBP4 (retinol binding protein 4).
