Human RBP4 ELISA Kit

$350.00$450.00

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TARGETSRBP4
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity1.05 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery88% – 110%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity1.05 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery88% – 110%
Average Recovery1.02
Intra Precision3.6% – 5.9%
Inter-Precision3.2% – 4.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-RBP4 monoclonal antibody
Human RBP4 freeze-dried standard
RBP4 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human RBP4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, RBP4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of RBP4 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

RBP4

RBP4 Target Infomation Overview

  • Target Symbol: RBP4, retinol binding protein 4
  • Gene Groups: Lipocalins
  • Alias Names: retinol-binding protein 4, plasma

RBP4, retinol binding protein 4 Target Infomation by Species

HumanMouseRat

Human RBP4 Target Information

  • Target Symbol: RBP4, retinol binding protein 4
  • Alias:
    • MCOPCB10
    • plasma retinol-binding protein
    • PRBP
    • RBP
    • RDCCAS
    • retinol binding protein 4, plasma
    • retinol-binding protein 4
    • retinol-binding protein 4, interstitial
  • NCBI_Gene: 5950
  • UniProtKB: P02753

Human RBP4 Predicted Functions

Enables retinol binding activity. Involved in several processes, including gluconeogenesis; positive regulation of insulin secretion; and retinol metabolic process. Located in extracellular space. Implicated in type 2 diabetes mellitus. Biomarker of type 2 diabetes mellitus.

Mouse Rbp4 Target Information

Mouse Rbp4 Predicted Functions

Enables retinol binding activity. Involved in several processes, including animal organ development; gluconeogenesis; and negative regulation of cardiac muscle cell proliferation. Acts upstream of or within several processes, including detection of light stimulus involved in visual perception; retinoid metabolic process; and terpenoid transport. Located in extracellular space. Is expressed in several structures, including adipose tissue; brain; genitourinary system; gut; and hindlimb. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human RBP4 (retinol binding protein 4).

Rat Rbp4 Target Information

Rat Rbp4 Predicted Functions

Enables retinol binding activity. Involved in response to ethanol and retinol transport. Located in extracellular space. Part of protein-containing complex. Biomarker of obesity and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in type 2 diabetes mellitus. Orthologous to human RBP4 (retinol binding protein 4).

RBP4 Target News

    Obesity Diabetes

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