Mouse CXCL10/IP-10 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CXCL10
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 50 μL；cell culture supernatant: 100 μL
Sensitivity	19.03 pg/mL
Range	312.5 pg/mL – 20000 pg/mL
Assay Time	3.5 h
Recovery	71% – 122%
Average Recovery	0.95

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 50 μL；cell culture supernatant: 100 μL
Sensitivity	19.03 pg/mL
Range	312.5 pg/mL – 20000 pg/mL
Assay Time	3.5 h
Recovery	71% – 122%
Average Recovery	0.95
Intra Precision	2.2% – 4.6%
Inter-Precision	1.3% – 3.8%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL10/IP-10 monoclonal antibody Mouse CXCL10/IP-10 freeze-dried standard CXCL10/IP-10 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IP-10 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IP-10 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IP-10 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

CXCL10

CXCL10 Target Infomation Overview

Target Symbol: CXCL10, C-X-C motif chemokine ligand 10
Gene Groups: Chemokine ligands
Alias: IFI10; IP-10; crg-2; mob-1; C7; gIP-10
Previous Names: INP10; SCYB10
Alias Names: small inducible cytokine subfamily B (Cys-X-Cys), member 10; chemokine (C-X-C motif) ligand 10

CXCL10, C-X-C motif chemokine ligand 10 Target Infomation by Species

HumanMouseRat

Human CXCL10 Target Information

Target Symbol: CXCL10, C-X-C motif chemokine ligand 10
Alias:
- 10 kDa interferon gamma-induced protein
- C-X-C motif chemokine 10
- C7
- chemokine (C-X-C motif) ligand 10
- crg-2
- gamma IP10
- gamma-IP10
- gIP-10
- IFI10
- INP10
- interferon-inducible cytokine IP-10
- IP-10
- mob-1
- protein 10 from interferon (gamma)-induced cell line
- SCYB10
- small inducible cytokine subfamily B (Cys-X-Cys), member 10
- small-inducible cytokine B10
NCBI_Gene: 3627
UniProtKB: P02778

Human CXCL10 Predicted Functions

Enables chemoattractant activity; chemokine receptor binding activity; and heparin binding activity. Involved in several processes, including positive regulation of release of sequestered calcium ion into cytosol; regulation of leukocyte chemotaxis; and response to virus. Acts upstream of or within cellular response to lipopolysaccharide and positive regulation of T cell migration. Located in extracellular region. Implicated in hepatitis B and middle cerebral artery infarction. Biomarker of several diseases, including artery disease (multiple); diabetes mellitus (multiple); fatty liver disease (multiple); liver disease (multiple); and lung disease (multiple).

Mouse Cxcl10 Target Information

Target Symbol: Cxcl10, chemokine (C-X-C motif) ligand 10
Alias:
- C7
- CRG-2
- gIP-10
- Ifi10
- INP10
- interferon activated gene 10
- IP-10
- IP10
- mob-1
- Scyb10
- small inducible cytokine B subfamily (Cys-X-Cys), member 10
NCBI_Gene: 15945

Mouse Cxcl10 Predicted Functions

Predicted to enable chemoattractant activity; chemokine receptor binding activity; and heparin binding activity. Acts upstream of or within several processes, including defense response to virus; negative regulation of myoblast differentiation; and negative regulation of myoblast fusion. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including alimentary system; axial skeleton; hemolymphoid system; and liver. Human ortholog(s) of this gene implicated in hepatitis B; middle cerebral artery infarction; and type 1 diabetes mellitus. Orthologous to human CXCL10 (C-X-C motif chemokine ligand 10).

Rat Cxcl10 Target Information

Target Symbol: Cxcl10, C-X-C motif chemokine ligand 10
Alias:
- 10 kDa interferon gamma-induced protein
- C-X-C motif chemokine 10
- chemokine (C-X-C motif) ligand 10
- gamma-IP10
- interferon-inducible cytokine IP-10
- interferon-inducible protein 10
- IP-10
- protein Mob-1
- Scyb10
- small inducible cytokine B subfamily (Cys-X-Cys) member 10
- small inducible cytokine B subfamily (Cys-X-Cys), member 10
- small-inducible cytokine B10
NCBI_Gene: 245920
UniProtKB: P48973

Rat Cxcl10 Predicted Functions

Enables chemokine activity. Involved in several processes, including response to auditory stimulus; response to gamma radiation; and response to vitamin D. Located in extracellular space. Used to study adult respiratory distress syndrome; myocarditis; proteinuria; renovascular hypertension; and type 1 diabetes mellitus. Biomarker of brain ischemia; encephalitis; and uveitis. Human ortholog(s) of this gene implicated in hepatitis B and middle cerebral artery infarction. Orthologous to human CXCL10 (C-X-C motif chemokine ligand 10).