Mouse FGF acidic ELISA Kit

$350.00$450.00

Download:
TARGETSFGF1
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.35 pg/mL
Range12.5 pg/mL – 800 pg/mL
Assay Time3.5 h
Recovery91% – 126%
Average Recovery1.09
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.35 pg/mL
Range12.5 pg/mL – 800 pg/mL
Assay Time3.5 h
Recovery91% – 126%
Average Recovery1.09
Intra Precision3.0% – 3.5%
Inter-Precision2.9% – 3.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-FGF acidic monoclonal antibody
Mouse FGF acidic freeze-dried standard
FGF acidic detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse FGF acidic antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, FGF acidic present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of FGF acidic in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

FGF1

FGF1 Target Infomation Overview

  • Target Symbol: FGF1, fibroblast growth factor 1
  • Gene Groups: Receptor ligands; Fibroblast growth factor family
  • Alias: AFGF; ECGF; ECGFA; ECGFB; HBGF1; ECGF-beta; FGF-alpha; GLIO703
  • Previous Names: FGFA
  • Alias Names: heparin-binding growth factor 1; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; fibroblast growth factor 1 (acidic)

FGF1, fibroblast growth factor 1 Target Infomation by Species

HumanMouseRat

Human FGF1 Target Information

  • Target Symbol: FGF1, fibroblast growth factor 1
  • Alias:
    • acidic fibroblast growth factor
    • AFGF
    • beta-endothelial cell growth factor
    • ECGF
    • ECGF-beta
    • ECGFA
    • ECGFB
    • endothelial cell growth factor
    • endothelial cell growth factor, alpha
    • endothelial cell growth factor, beta
    • FGF-1
    • FGF-alpha
    • FGFA
    • fibroblast growth factor 1 (acidic)
    • GLIO703
    • HBGF-1
    • HBGF1
    • heparin-binding growth factor 1
  • NCBI_Gene: 2246
  • UniProtKB: P05230

Human FGF1 Predicted Functions

Enables S100 protein binding activity; heparin binding activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of MAPK cascade; positive regulation of biosynthetic process; and regulation of endothelial cell migration. Located in cytosol; extracellular space; and nucleoplasm. Implicated in brain ischemia; cleft lip; myocardial infarction; schizophrenia; and urinary bladder cancer. Biomarker of Alzheimer’s disease; endometrial cancer; leiomyoma; ovarian cancer; and transitional cell carcinoma.

Mouse Fgf1 Target Information

Mouse Fgf1 Predicted Functions

Predicted to enable several functions, including Hsp70 protein binding activity; S100 protein binding activity; and signaling receptor binding activity. Acts upstream of or within several processes, including organ induction; positive regulation of epithelial cell proliferation; and positive regulation of nitrogen compound metabolic process. Located in extracellular matrix and extracellular space. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in brain ischemia; cleft lip; myocardial infarction; schizophrenia; and urinary bladder cancer. Orthologous to human FGF1 (fibroblast growth factor 1).

Rat Fgf1 Target Information

  • Target Symbol: Fgf1, fibroblast growth factor 1
  • Alias:
    • acidic fibroblast growth factor
    • aFGF
    • FGF-1
    • Fgf2b
    • fibroblast growth factor 1 (acidic)
    • Fibroblast growth factor 1 (heparin binding)
    • fibroblast growth factor 2b
    • HBGF-1
    • HBGF1
    • heparin-binding growth factor 1
  • NCBI_Gene: 25317

Rat Fgf1 Predicted Functions

Enables Hsp70 protein binding activity and growth factor activity. Involved in positive regulation of hepatocyte proliferation. Located in cytoplasm and nucleolus. Used to study brain ischemia. Human ortholog(s) of this gene implicated in brain ischemia; cleft lip; myocardial infarction; schizophrenia; and urinary bladder cancer. Orthologous to human FGF1 (fibroblast growth factor 1).

FGF1 Target News

    Related Products

    more +

    EK1392

    $350.00$450.00

    EK1341

    $350.00$450.00

    EK1C01

    $350.00$450.00

    EK1242

    $350.00$450.00

    EK1334

    $350.00$450.00

    EK2295

    $350.00$450.00

    EK1360

    $350.00$450.00

    EK2100

    $350.00$450.00

    $350.00$450.00

    EK1349

    $350.00$450.00

    $350.00$450.00

    $350.00$450.00

    EK2153

    $350.00$450.00

    $350.00$450.00

    EK2F01

    $350.00$450.00

    EK2313

    $350.00$450.00

    Same Target

    EK1285

    $350.00$450.00