EK2295

Mouse IGFBP-5 ELISA Kit

$245.00$350.00

Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity1.73 pg/mL
Array Range62.50 - 4000 pg/mL
Assay Time3.5 h
Recovery93% - 110%
Average Recovery100%

ELISA Kit Detail Information

Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity1.73 pg/mL
Array Range62.50 - 4000 pg/mL
Assay Time3.5 h
Recovery93% - 110%
Average Recovery100%
Intra Precision2.65% - 4.80%
Inter Precision3.79% - 5.30%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
ComponentsIGFBP-5 Microplate
IGFBP-5 Standard
IGFBP-5 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer
Substrate
Stop Solution
Washing Buffer
Plate Covers
Assay PrincipleThis kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-mouse IGFBP-5 antibodies are precoated on a high-affinity ELISA plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the plate and incubated, allowing the IGFBP-5 in the samples to bind to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, HRP-labeled streptavidin is added. After washing again, a chromogenic substrate TMB is added for color development under light protection. The intensity of the color reaction is directly proportional to the concentration of IGFBP-5 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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