Mouse IL-33 ELISA Kit

$350.00$450.00

TARGETS IL33
SPECIES Mouse
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume Serum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity 8.74 pg/mL
Range 46.88 pg/mL – 3000 pg/mL
Assay Time 3.5 h
Recovery 85% – 117%
Average Recovery 0.98
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume Serum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity 8.74 pg/mL
Range 46.88 pg/mL – 3000 pg/mL
Assay Time 3.5 h
Recovery 85% – 117%
Average Recovery 0.98
Intra Precision 4.9% – 7.2%
Inter-Precision 4.9% – 8.1%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-33 monoclonal antibody
Mouse IL-33 freeze-dried standard
IL-33 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-33 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-33 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-33 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IL33

IL33 Target Infomation Overview

  • Target Symbol: IL33, interleukin 33
  • Gene Groups: Interleukins
  • Alias: DVS27; DKFZp586H0523; NF-HEV; IL1F11
  • Previous Names: C9orf26
  • Alias Names: DVS27-related protein; nuclear factor for high endothelial venules; interleukin-1 family, member 11; chromosome 9 open reading frame 26 (NF-HEV)

IL33, interleukin 33 Target Infomation by Species

[su_tabs][su_tab title=”Human” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-human”]

Human IL33 Target Information

  • Target Symbol: IL33, interleukin 33
  • Alias:
    • C9orf26
    • DKFZp586H0523
    • DVS27
    • DVS27-related protein
    • IL-1F11
    • IL-33
    • IL1F11
    • interleukin-1 family member 11
    • interleukin-33
    • NF-HEV
    • NFEHEV
    • nuclear factor for high endothelial venules
    • nuclear factor from high endothelial venules
    • RP11-575C20.2
  • NCBI_Gene: 90865
  • UniProtKB: O95760

Human IL33 Predicted Functions

Enables cytokine activity. Involved in negative regulation of transcription by RNA polymerase II; positive regulation of chemokine production; and positive regulation of macrophage activation. Located in nucleoplasm. Part of cytoplasm and nucleus. Implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Biomarker of several diseases, including colitis (multiple); common cold; gout; liver disease (multiple); and tuberculosis (multiple).

[/su_tab]
[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Il33 Target Information

Mouse Il33 Predicted Functions

Enables cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including interleukin-33-mediated signaling pathway; microglial cell activation involved in immune response; and regulation of gene expression. Acts upstream of or within several processes, including negative regulation of macrophage proliferation; positive regulation of cellular defense response; and positive regulation of macromolecule metabolic process. Located in nucleus. Is expressed in several structures, including adrenal gland; alimentary system; genitourinary system; nervous system; and sensory organ. Used to study Alzheimer’s disease. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).

[/su_tab]
[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Il33 Target Information

Rat Il33 Predicted Functions

Predicted to enable cytokine activity and interleukin-33 receptor binding activity. Involved in several processes, including cellular response to mechanical stimulus; positive regulation of oligodendrocyte differentiation; and regulation of sensory perception of pain. Located in nucleus. Biomarker of acute myocardial infarction; colon cancer; and diabetes mellitus. Human ortholog(s) of this gene implicated in candidiasis; inflammatory bowel disease; and peptic ulcer disease. Orthologous to human IL33 (interleukin 33).

[/su_tab][/su_tabs]

IL33 Target News

[catlist tags=”il33″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

3.9
Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis
Frontiers in Molecular Biosciences
4.3
Celastrol alleviates atopic dermatitis by regulating Ezrin-mediated mitochondrial fission and fusion
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
4.8
Jiawei Yanghe Decoction attenuate allergic airway inflammation by suppressing group 2 innate lymphoid cells responses
JOURNAL OF ETHNOPHARMACOLOGY
10
Ultrasmall Nanoparticles Regulate Immune Microenvironment by Activating IL-33/ST2 to Alleviate Renal Ischemia-Reperfusion Injury
Advanced Healthcare Materials
4.8
Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance
INTERNATIONAL IMMUNOPHARMACOLOGY
3
Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines
Balkan Medical Journal
9
Honokiol suppresses the aberrant interactions between renal resident macrophages and tubular epithelial cells in lupus nephritis through the NLRP3/IL-33/ST2 axis
Cell Death & Disease
2.4
The egg ribonuclease SjCP1412 accelerates liver fibrosis caused by Schistosoma japonicum infection involving damage associated molecular patterns (DAMPs)
PARASITOLOGY
5.4
Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice
JOURNAL OF ETHNOPHARMACOLOGY
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Lung Disease (Asthma & COPD)
Osteoarthrosis & Rheumatoid Arthritis
Systemic Lupus Erythematosus

Related Products

more +

EK1392

$350.00$450.00

EK1341

$350.00$450.00

EK1C01

$350.00$450.00

EK1242

$350.00$450.00

EK1334

$350.00$450.00

EK2295

$350.00$450.00

EK1360

$350.00$450.00

EK2100

$350.00$450.00

EK224

$350.00$450.00

EK1349

$350.00$450.00

EK397

$350.00$450.00

EK285

$350.00$450.00

EK2153

$350.00$450.00

EK387

$350.00$450.00

EK2F01

$350.00$450.00

EK2313

$350.00$450.00

Same Target

$380.00$480.00