Human Bcl-2 ELISA Kit

$350.00$450.00

TARGETS BCL2
SPECIES Human
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 80 μL 1×Assay Buffer and 20 μL sample
Sensitivity 50.25 pg/mL
Range 0.47 ng/mL – 30 ng/mL
Assay Time 3.5 h
Recovery 90% – 110%
Average Recovery 0.99
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 80 μL 1×Assay Buffer and 20 μL sample
Sensitivity 50.25 pg/mL
Range 0.47 ng/mL – 30 ng/mL
Assay Time 3.5 h
Recovery 90% – 110%
Average Recovery 0.99
Intra Precision 4.0% – 4.9%
Inter-Precision 2.4% – 4.7%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Bcl-2 monoclonal antibody
Human Bcl-2 freeze-dried standard
Bcl-2 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Bcl-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, Bcl-2 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Bcl-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

BCL2

BCL2 Target Infomation Overview

  • Target Symbol: BCL2, BCL2 apoptosis regulator
  • Gene Groups: Protein phosphatase 1 regulatory subunits; BCL2 family
  • Alias: Bcl-2; PPP1R50
  • Alias Names: protein phosphatase 1, regulatory subunit 50; B-cell CLL/lymphoma 2; BCL2, apoptosis regulator

BCL2, BCL2 apoptosis regulator Target Infomation by Species

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Human BCL2 Target Information

  • Target Symbol: BCL2, BCL2 apoptosis regulator
  • Alias:
    • apoptosis regulator Bcl-2
    • B-cell CLL/lymphoma 2
    • b-cell leukemia/lymphoma 2
    • Bcl-2
    • Bcl-2a
    • Bcl-2ß
    • BCL2, apoptosis regulator
    • PPP1R50
    • protein phosphatase 1, regulatory subunit 50
  • NCBI_Gene: 596
  • UniProtKB: P10415

Human BCL2 Predicted Functions

Enables several functions, including BH3 domain binding activity; enzyme binding activity; and protein heterodimerization activity. Involved in several processes, including B cell receptor signaling pathway; negative regulation of apoptotic process; and positive regulation of B cell proliferation. Acts upstream of or within several processes, including B cell proliferation; apoptotic signaling pathway; and negative regulation of apoptotic process. Located in endoplasmic reticulum; mitochondrial outer membrane; and nucleus. Part of pore complex. Implicated in several diseases, including carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; prostate cancer; and urinary bladder cancer. Biomarker of several diseases, including cervix uteri carcinoma in situ; ductal carcinoma in situ; gastrointestinal system cancer (multiple); hematologic cancer (multiple); and urinary system cancer (multiple).

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Mouse Bcl2 Target Information

Mouse Bcl2 Predicted Functions

Enables protein phosphatase 2A binding activity. Involved in several processes, including hair follicle morphogenesis; mitochondrion organization; and negative regulation of reactive oxygen species metabolic process. Acts upstream of or within several processes, including animal organ development; apoptotic signaling pathway; and negative regulation of programmed cell death. Located in several cellular components, including mitochondrial membrane; myelin sheath; and nuclear membrane. Is expressed in several structures, including central nervous system; genitourinary system; gut; limb; and sensory organ. Used to study oligomeganephronia. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease; carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; and prostate cancer. Orthologous to human BCL2 (BCL2 apoptosis regulator).

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Rat Bcl2 Target Information

Rat Bcl2 Predicted Functions

Enables BH domain binding activity. Involved in several processes, including response to peptide; response to purine-containing compound; and response to vitamin. Located in cytosol; mitochondrial crista; and perinuclear region of cytoplasm. Used to study several diseases, including brain ischemia (multiple); end stage renal disease; gastric ulcer; intestinal disease (multiple); and status epilepticus. Biomarker of several diseases, including abdominal obesity-metabolic syndrome 1; brain disease (multiple); hypertension (multiple); intrinsic cardiomyopathy (multiple); and neurodegenerative disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; prostate cancer; and urinary bladder cancer. Orthologous to human BCL2 (BCL2 apoptosis regulator).

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BCL2 Target News

[catlist tags=”bcl2″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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