Human Bcl-2 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	BCL2
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	80 μL 1×Assay Buffer and 20 μL sample
Sensitivity	50.25 pg/mL
Range	0.47 ng/mL – 30 ng/mL
Assay Time	3.5 h
Recovery	90% – 110%
Average Recovery	0.99

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	80 μL 1×Assay Buffer and 20 μL sample
Sensitivity	50.25 pg/mL
Range	0.47 ng/mL – 30 ng/mL
Assay Time	3.5 h
Recovery	90% – 110%
Average Recovery	0.99
Intra Precision	4.0% – 4.9%
Inter-Precision	2.4% – 4.7%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Bcl-2 monoclonal antibody Human Bcl-2 freeze-dried standard Bcl-2 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Bcl-2 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, Bcl-2 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Bcl-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

BCL2

BCL2 Target Infomation Overview

Target Symbol: BCL2, BCL2 apoptosis regulator
Gene Groups: Protein phosphatase 1 regulatory subunits; BCL2 family
Alias: Bcl-2; PPP1R50
Alias Names: protein phosphatase 1, regulatory subunit 50; B-cell CLL/lymphoma 2; BCL2, apoptosis regulator

BCL2, BCL2 apoptosis regulator Target Infomation by Species

HumanMouseRat

Human BCL2 Target Information

Target Symbol: BCL2, BCL2 apoptosis regulator
Alias:
- apoptosis regulator Bcl-2
- B-cell CLL/lymphoma 2
- b-cell leukemia/lymphoma 2
- Bcl-2
- Bcl-2a
- Bcl-2ß
- BCL2, apoptosis regulator
- PPP1R50
- protein phosphatase 1, regulatory subunit 50
NCBI_Gene: 596
UniProtKB: P10415

Human BCL2 Predicted Functions

Enables several functions, including BH3 domain binding activity; enzyme binding activity; and protein heterodimerization activity. Involved in several processes, including B cell receptor signaling pathway; negative regulation of apoptotic process; and positive regulation of B cell proliferation. Acts upstream of or within several processes, including B cell proliferation; apoptotic signaling pathway; and negative regulation of apoptotic process. Located in endoplasmic reticulum; mitochondrial outer membrane; and nucleus. Part of pore complex. Implicated in several diseases, including carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; prostate cancer; and urinary bladder cancer. Biomarker of several diseases, including cervix uteri carcinoma in situ; ductal carcinoma in situ; gastrointestinal system cancer (multiple); hematologic cancer (multiple); and urinary system cancer (multiple).

Mouse Bcl2 Target Information

Target Symbol: Bcl2, B cell leukemia/lymphoma 2
Alias:
- AW986256
- Bcl-2
- C430015F12Rik
- D630044D05Rik
- D830018M01Rik
- expressed sequence AW986256
- RIKEN cDNA C430015F12 gene
- RIKEN cDNA D630044D05 gene
- RIKEN cDNA D830018M01 gene
NCBI_Gene: 12043

Mouse Bcl2 Predicted Functions

Enables protein phosphatase 2A binding activity. Involved in several processes, including hair follicle morphogenesis; mitochondrion organization; and negative regulation of reactive oxygen species metabolic process. Acts upstream of or within several processes, including animal organ development; apoptotic signaling pathway; and negative regulation of programmed cell death. Located in several cellular components, including mitochondrial membrane; myelin sheath; and nuclear membrane. Is expressed in several structures, including central nervous system; genitourinary system; gut; limb; and sensory organ. Used to study oligomeganephronia. Human ortholog(s) of this gene implicated in several diseases, including Alzheimer’s disease; carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; and prostate cancer. Orthologous to human BCL2 (BCL2 apoptosis regulator).

Rat Bcl2 Target Information

Target Symbol: Bcl2, BCL2, apoptosis regulator
Alias:
- apoptosis regulator Bcl-2
- B cell lymphoma 2 associated oncogene
- B-cell CLL/lymphoma 2
- B-cell leukemia/lymphoma 2
- Bcl-2
- Bcl-2alpha
- Bcl2-like protein
NCBI_Gene: 24224

Rat Bcl2 Predicted Functions

Enables BH domain binding activity. Involved in several processes, including response to peptide; response to purine-containing compound; and response to vitamin. Located in cytosol; mitochondrial crista; and perinuclear region of cytoplasm. Used to study several diseases, including brain ischemia (multiple); end stage renal disease; gastric ulcer; intestinal disease (multiple); and status epilepticus. Biomarker of several diseases, including abdominal obesity-metabolic syndrome 1; brain disease (multiple); hypertension (multiple); intrinsic cardiomyopathy (multiple); and neurodegenerative disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); hematologic cancer (multiple); intracranial aneurysm; prostate cancer; and urinary bladder cancer. Orthologous to human BCL2 (BCL2 apoptosis regulator).